Aro Andrea A, Vidal Benedicto C, Tomiosso Tatiana C, Gomes Laurecir, Matiello-Rosa Stella M G, Pimentel Edson R
Department of Cell Biology, State University of Campinas, Campinas, SP, Brazil.
Connect Tissue Res. 2008;49(6):443-54. doi: 10.1080/03008200802325250.
Little is known about the stretching effects on the biochemical and morphological features of tendons submitted to a long period of immobilization. Our purpose was to evaluate the response of rat tendons to stretching procedures after immobilization. The animals were separated into five experimental groups: GI--control of immobilized and euthanized animals; GII--immobilized and euthanized animals; GIII--control of immobilized animals and afterward stretched or allowed free cage activity; GIV--immobilized and stretched animals; and GV--immobilized and allowed free cage activity. Analysis in SDS-PAGE showed no remarkable differences among the groups, but a prominent collagen band was observed in GV, as compared to GIV and the control group, both in the compression and tension regions. Hydroxyproline content was highest in the compression region of GII. No differences among the groups were observed in the tension region. In regard to the concentration of noncollagenous proteins, differences were detected only in the tension region, where larger concentrations were found in the GII. When GII and GIV were compared, highest values were found in the GII. A more abundant presence of sulfated glycosaminoglycans, especially chondroitin sulfate, was detected in GIV, at the compression region of tendons. The presence of dermatan sulfate was outstanding in the compression and tension regions of the GII and GV groups. In the Ponceau SS stained sections, analyzed under polarization microscopy, GII exhibited the highest disorganization of the collagen bundles, partially recovered after stretching or with only remobilization. Our results indicate that a revision in the stretching procedures, in terms of duration and periodicity of the sessions, could benefit the efficiency of the stretching in cases of previous immobilization of tendons.
关于长时间固定对肌腱生化和形态特征的拉伸影响，人们了解甚少。我们的目的是评估大鼠肌腱在固定后对拉伸程序的反应。将动物分为五个实验组：GI组——固定并安乐死动物的对照组；GII组——固定并安乐死动物；GIII组——固定动物的对照组，随后进行拉伸或允许其在笼内自由活动；GIV组——固定并拉伸的动物；GV组——固定并允许在笼内自由活动。SDS - PAGE分析显示各组之间没有显著差异，但与GIV组和对照组相比，GV组在压缩区和张力区均观察到一条明显的胶原带。羟脯氨酸含量在GII组的压缩区最高。在张力区未观察到各组之间的差异。关于非胶原蛋白的浓度，仅在张力区检测到差异，GII组的浓度更高。当比较GII组和GIV组时，GII组的值最高。在肌腱的压缩区，GIV组检测到更丰富的硫酸化糖胺聚糖，尤其是硫酸软骨素。硫酸皮肤素在GII组和GV组的压缩区和张力区含量显著。在经丽春红S染色的切片中，在偏光显微镜下分析，GII组的胶原束紊乱程度最高，在拉伸或仅重新活动后部分恢复。我们的结果表明，在拉伸程序的持续时间和周期方面进行调整，可能有利于在先前肌腱固定的情况下提高拉伸效率。
