Genetic polymorphism at the candidate gene in Iranian Sistani cattle (Bos indicus).

The genotypes for Leptin, Kappa-Casein, Calpastatin and BoLA-DRB3 loci were determined by polymerase chain reaction and restriction enzyme digestion method in native Iranian breed cattle, Sistani. Blood samples were collected from Sistani Breeding Station located in Zehak, Zabol in Iran. The extraction of genomic DNA was based on Guanidin Thiocyanate-Silica gel method. After PCR reaction, amplicons were digested with appropriate restriction enzymes. The Calpastatin locus had 3 genotypes with frequencies of 0.62, 0.29 and 0.09 for MM, MN and NN, respectively; kappa-Casein and Leptin had 3 genotypes with frequencies of 0.27, 0.57 and 0.16 for kappa-Casein, 0.77, 0.22 and 0.01 for Leptin for AA, AB and BB genotypes, respectively. For BoLA-DRB3 we identified 19 alleles, that DRB3. 28 had the highest allelic frequency (22.4%) and DRB3. 23, *29, *37 and *51 had the lowest allelic frequency (1%). One of the 19 alleles had a new pattern. Average heterozygosity values for all loci were low. Chi2-test did not confirm the Hardy-Weinberg equilibrium for Leptin and Calpastatin in this population. These data provide evidence that Iranian's Sistani breed have a good genetic variability, which opens interesting prospects for future selection programs, especially marker-assistant selection.