伊朗加兹温省牛隐孢子虫的流行情况及分子特征
Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran.
作者信息
Keshavarz Akbar, Haghighi Ali, Athari Amid, Kazemi Bahram, Abadi Alireza, Nazemalhosseini Mojarad Ehsan
机构信息
Department of Medical Parasitology & Mycology, Shahid Beheshti University, M.C., Tehran, Iran.
出版信息
Vet Parasitol. 2009 Mar 23;160(3-4):316-8. doi: 10.1016/j.vetpar.2008.11.008. Epub 2008 Nov 13.
The aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of Cryptosporidium in cattle from 15 dairy farms in Qazvin province, Iran. Fecal samples, collected from 272 cattle during May 2006 to December 2007, were characterized microscopically. Oocysts from 51 positive samples were analyzed using PCR assay of 18S SSU rRNA, restriction fragment length polymorphism (RFLP) and sequencing. We identified 72.6% of the positive samples as Cryptosporidium parvum, 17.7% as Cryptosporidium andersoni, 7.8% as Cryptosporidium bovis and 1.9% as a novel genotype of C. parvum possessing a single mutation on MboII restriction. An infection rate of 19.5% of C. parvum among 174 pre-weaned calves was significantly higher than the 3.1% among 98 post-weaned calves (P<0.0006). This is the first report of C. bovis and the new subgenotype of C. parvum in Iranian cattle.
本研究旨在确定伊朗加兹温省15个奶牛场牛群中隐孢子虫的流行情况、随宿主年龄的变化以及其种类的基因型。2006年5月至2007年12月期间从272头牛采集的粪便样本进行了显微镜检查。对51份阳性样本的卵囊进行了18S SSU rRNA的聚合酶链反应（PCR）分析、限制性片段长度多态性（RFLP）分析和测序。我们将72.6%的阳性样本鉴定为微小隐孢子虫，17.7%为安氏隐孢子虫，7.8%为牛隐孢子虫，1.9%为微小隐孢子虫的一种新型基因型，其在MboII限制性位点有一个单突变。174头断奶前犊牛中微小隐孢子虫的感染率为19.5%，显著高于98头断奶后犊牛中的3.1%（P<0.0006）。这是伊朗牛群中牛隐孢子虫和微小隐孢子虫新亚型的首次报告。
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