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卡诺棕山羊胎儿及成年卵巢形态学特征的研究。

Studies on morphological features of foetal and adult ovaries in Kano brown goats.

作者信息

Chima Nwaogu Innocent, Ifeoma Okolie Pauline

机构信息

Department of Veterinary Anatomy, University of Nigeria, Nsukka, Enugu State, Nigeria.

出版信息

Anim Reprod Sci. 2009 Oct;115(1-4):58-65. doi: 10.1016/j.anireprosci.2008.10.023. Epub 2008 Nov 11.

Abstract

In this work we studied the structures of 51 foetal and 14 adult ovaries obtained from slaughtered Kano brown does in Nsukka abattoir. The ages of the adult does were determined by dentition and foetuses by crown rump length method. The foetal and adult ovaries were divided into five different groups using specific age intervals as Gestation day (GD) 50-65, 66-95, 96-125 and 126-145 and adults. For histological studies the ovaries were fixed, processed and routinely stained with H&E. The ovarian follicles were classified into 5 types according to granulosa cell layers surrounding the oocytes. The number of ovarian follicles per microscopic field, number of granulosa cells surrounding type 1 and 1A follicles and diameter of the ovarian follicles were determined for each group at 400x magnification. Grossly the foetal ovaries were like pin head, oval in shape, uniformly smooth and creamy in colour. The adult ovaries had follicles with different sizes. The adult mean ovarian weights were significantly higher (P<0.01) than those of the foetuses. Microscopically, the GD 50-65 ovaries had no distinct cortex and medulla. Oogonia were numerous among other stromal cells toward the periphery of the ovary. By GD 66-95 the ovaries contained types 1, 1a, 2 and 3 follicles. GD 96-125 ovaries contained type 4 follicles with early antrum formation and those of GD 126-145 comprised type 5 among other follicles. The adult ovaries comprised all the ovarian follicle types. The number of type 1 follicles increased significantly (P<0.01) with foetal age. It was least in the adults. The diameter of adult follicles was significantly higher (P<0.01) than those of the foetuses. This result provides baseline information on the morphological development of ovaries in Kano brown goats.

摘要

在这项研究中,我们对从Nsukka屠宰场宰杀的卡诺棕山羊获取的51个胎儿卵巢和14个成年卵巢的结构进行了研究。成年山羊的年龄通过牙齿状况确定,胎儿的年龄通过冠臀长法确定。根据特定的年龄区间,将胎儿和成年卵巢分为五个不同的组,即妊娠第50 - 65天、66 - 95天、96 - 125天、126 - 145天的胎儿组以及成年组。为进行组织学研究,将卵巢固定、处理并常规用苏木精和伊红染色。根据围绕卵母细胞的颗粒细胞层,将卵巢卵泡分为5种类型。在400倍放大倍数下,测定每组每个显微镜视野中的卵巢卵泡数量、1型和1A型卵泡周围的颗粒细胞数量以及卵巢卵泡的直径。大体上,胎儿卵巢如针头大小,呈椭圆形,表面均匀光滑,颜色呈奶油色。成年卵巢有大小不同的卵泡。成年卵巢的平均重量显著高于胎儿卵巢(P<0.01)。在显微镜下,妊娠第50 -

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