Esheba Ghada E, Longacre Teri A, Atkins Kristen A, Higgins John P
Department of Pathology, Stanford University, Stanford, CA 94305, USA.
Am J Surg Pathol. 2009 Mar;33(3):347-53. doi: 10.1097/PAS.0b013e3181908e24.
The degree of urothelial differentiation in putative transitional (urothelial) proliferations in the female genital tract is still controversial. To further investigate the similarities (or dissimilarities) between female genital tract transitional proliferations and bladder urothelium, we evaluated the expression of S100P and GATA3, 2 proteins that we previously found to be strongly expressed in bladder urothelial tumors, in 25 benign ovarian Brenner tumors, 19 Walthard cell nests (17 tubal and 2 ovarian hilus), 1 mature teratoma with a benign urothelial proliferation, 2 proliferating (borderline) ovarian Brenner tumors, 1 malignant Brenner tumor, and 12 ovarian transitional cell carcinomas (TCC). Each lesion was also evaluated for p63 expression by immunohistochemistry. Immunostaining was performed on formalin-fixed, paraffin-embedded tissue sections using the avidin-biotin-peroxidase complex method. Eighty-eight percent of Brenner tumors were positive for S100P, whereas 96% and 100% were positive for GATA3 and p63, respectively. One of 2 proliferating Brenner tumors was positive for S100P, whereas both cases were positive for GATA3 and p63; the malignant Brenner tumor was positive for S100P and p63, but negative for GATA3. Only 17% of TCC were positive for S100p, whereas 33% and 50% of TCC were positive for GATA3 and p63, respectively. Tubal Walthard cell nests were either completely negative or showed only scattered positive staining for S100P; in contrast, 89.5% and 100% of Walthard nests, including the 2 ovarian cases were positive for GATA3 and p63. The teratoma-associated benign urothelial proliferation was also negative for S100P, but positive for GATA3 and p63. Although proliferating and malignant Brenner tumors may exhibit a more intermediate immunoprofile, expression of S100P, GATA3, and p63 by a majority of ovarian Brenner tumors underscores the similarity between these neoplasms and urothelial proliferations of bladder origin. The indeterminate phenotype seen in Walthard nests and ovarian TCC suggests that these proliferations may represent an incomplete or alternate form of differentiation.
女性生殖道中假定的移行（尿路上皮）增殖的尿路上皮分化程度仍存在争议。为了进一步研究女性生殖道移行增殖与膀胱尿路上皮之间的相似性（或差异），我们评估了S100P和GATA3这两种蛋白的表达，我们之前发现它们在膀胱尿路上皮肿瘤中强烈表达，研究对象包括25例良性卵巢布伦纳瘤、19个瓦尔塔德细胞巢（17个输卵管的和2个卵巢门的）、1例伴有良性尿路上皮增殖的成熟畸胎瘤、2例增殖性（交界性）卵巢布伦纳瘤、1例恶性布伦纳瘤和12例卵巢移行细胞癌（TCC）。通过免疫组织化学对每个病变的p63表达也进行了评估。使用抗生物素蛋白-生物素-过氧化物酶复合物方法对福尔马林固定、石蜡包埋的组织切片进行免疫染色。88%的布伦纳瘤S100P呈阳性，而GATA3和p63的阳性率分别为96%和100%。2例增殖性布伦纳瘤中有1例S100P呈阳性，而2例GATA3和p63均呈阳性；恶性布伦纳瘤S100P和p63呈阳性，但GATA3呈阴性。只有17%的TCC S100p呈阳性，而GATA3和p63的阳性率分别为33%和50%。输卵管瓦尔塔德细胞巢S100P要么完全阴性，要么仅显示散在的阳性染色；相比之下，89.5%和100%的瓦尔塔德巢（包括2例卵巢的）GATA3和p63呈阳性。畸胎瘤相关的良性尿路上皮增殖S100P也呈阴性，但GATA3和p63呈阳性。虽然增殖性和恶性布伦纳瘤可能表现出更中间的免疫表型，但大多数卵巢布伦纳瘤中S100P、GATA3和p63的表达强调了这些肿瘤与膀胱起源的尿路上皮增殖之间的相似性。在瓦尔塔德巢和卵巢TCC中看到的不确定表型表明，这些增殖可能代表一种不完全或替代的分化形式。
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