University of Physical Education Poznan, Faculty of Physical Culture Gorzow Wlkp., Department of Biochemistry, Gorzow Wlkp., Poland.
Physiol Res. 2009;58(6):855-861. doi: 10.33549/physiolres.931590. Epub 2008 Dec 17.
The aim of this study was to follow up whether the modification of pro-antioxidant status by oral thiol administration such as N-acetylcysteine and alpha-lipoic acid affects the hematological response. Twenty-eight healthy men participated in two independent experiments. Subjects were randomly assigned to one of four groups: controls (C(NAC) and C(ALA)), N-acetylcysteine (NAC) and alpha-lipoic acid (ALA). 1200 mg of N-acetylcysteine, 600 mg of alpha-lipoic acid or placebo were administered for 8 days in two doses. NAC or ALA administration significantly elevated plasma total antioxidant status (TAS) and reduced protein carbonylation (PC) and lipid peroxidation (TBARS) by more than 30 %. The reduced glutathione (GSH) and hematological parameters changed only in response to NAC administration. NAC significantly elevated the level of GSH (+33%), EPO (+26%), Hb (+9%) and Hct (+9%) compared with C(NAC). The mean corpuscular volume (MCV) and the mean corpuscular hemoglobin (MCH) also increased by more than 12% after NAC. The numerous negative or positive correlations between the measures of TAS, PC, TBARS and hematological parameters were found, which suggest the NAC-induced interaction between pro-antioxidant and hematological values. Our study has shown that both N-acetylcysteine and alpha-lipoic acid intake reveal an antioxidant action, but only N-acetylcysteine improves the haematological response.
本研究旨在观察口服巯基供体如 N-乙酰半胱氨酸（NAC）和α-硫辛酸（ALA）对前抗氧化状态的修饰是否影响血液学反应。28 名健康男性参与了两项独立的实验。受试者被随机分配到以下四组之一：对照组（C（NAC）和 C（ALA））、N-乙酰半胱氨酸（NAC）和α-硫辛酸（ALA）。1200mg NAC、600mg ALA 或安慰剂以 2 次剂量给药 8 天。NAC 或 ALA 给药可使血浆总抗氧化状态（TAS）显著升高，并使蛋白羰基化（PC）和脂质过氧化（TBARS）降低 30%以上。只有 NAC 给药可使还原型谷胱甘肽（GSH）和血液学参数发生变化。与 C（NAC）相比，NAC 给药可使 GSH 水平升高（+33%）、EPO（+26%）、Hb（+9%）和 Hct（+9%）。NAC 给药后，平均红细胞体积（MCV）和平均红细胞血红蛋白（MCH）也增加了 12%以上。TAS、PC、TBARS 和血液学参数之间存在许多负相关或正相关，这表明 NAC 诱导的前抗氧化剂和血液学值之间的相互作用。本研究表明，NAC 和 ALA 摄入均具有抗氧化作用，但只有 NAC 可改善血液学反应。
