Modo Michel, Meade Thomas J, Mitry Ragai R
Centre for the Cellular Basis of Behaviour, Institute of Psychiatry, King's College London, UK.
Methods Mol Biol. 2009;481:207-19. doi: 10.1007/978-1-59745-201-4_17.
Cell transplantation is a promising approach to improve the life of patients with liver disease. At present, however, techniques to track and visualise transplanted cells in patients are fairly limited and further development of non-invasive imaging technology is needed to advance the monitoring of liver cell grafts. Magnetic resonance imaging (MRI) is a non-invasive imaging technology that already allows the visualisation of particular cell fractions in the liver by using MR contrast agents. The use of contrast agents to pre-label liver cells prior to transplantation will potentially provide a method to identify, track and study the integration of engrafted cells non-invasively by MRI. Before this technique can find its clinical application, in vitro and pre-clinical in vivo studies need to be conducted to determine the safety and specificity of this approach.
细胞移植是改善肝病患者生活的一种有前景的方法。然而，目前在患者体内追踪和可视化移植细胞的技术相当有限，需要进一步开发非侵入性成像技术来推进对肝细胞移植的监测。磁共振成像（MRI）是一种非侵入性成像技术，已经可以通过使用磁共振造影剂来可视化肝脏中的特定细胞成分。在移植前使用造影剂对肝细胞进行预标记，有可能提供一种通过MRI非侵入性地识别、追踪和研究植入细胞整合情况的方法。在该技术能够应用于临床之前，需要进行体外和临床前体内研究，以确定这种方法的安全性和特异性。
