Kadurugamuwa J L, Rohde M, Wehland J, Timmis K N
Department of Microbiology, GBF-National Research Center for Biotechnology, Braunschweig, Germany.
Infect Immun. 1991 Oct;59(10):3463-71. doi: 10.1128/iai.59.10.3463-3471.1991.
The spread of Shigella flexneri in a monolayer of infected Henle and HeLa cells was studied by using immunofluorescence and electron microscopy. Infected cells produced numerous bacterium-containing membranous protrusions up to 18 microns in length that penetrated adjacent cells and were subsequently phagocytosed. Fluorescence staining of actin and vinculin in infected cells with phalloidin and monoclonal antibody to vinculin, respectively, demonstrated that the protrusions containing the bacteria consisted of these cytoskeletal proteins. Actin accumulated predominantly at the poles of bacteria distal to the tip of protrusions and appeared as trails extending back towards the host cell cytoplasm. Vinculin, however, was distributed uniformly around the bacteria and throughout the protrusion. A profound rearrangement of vinculin occurred in Henle and HeLa cells following infection with shigellae: whereas in uninfected cells it was distributed mainly around the cell periphery, in infected cells it concentrated mainly around clusters of bacteria in the cytoplasm. This suggests a possible involvement of the vinculin cytoskeletal protein in the intercellular spread of shigellae during an infection.
通过免疫荧光和电子显微镜研究了福氏志贺菌在感染的Henle细胞和HeLa细胞单层中的传播情况。受感染的细胞产生了许多含细菌的膜性突起，长度可达18微米，这些突起穿透相邻细胞并随后被吞噬。分别用鬼笔环肽和抗纽蛋白单克隆抗体对受感染细胞中的肌动蛋白和纽蛋白进行荧光染色，结果表明含有细菌的突起由这些细胞骨架蛋白组成。肌动蛋白主要聚集在突起尖端远端的细菌两极，并表现为向宿主细胞质延伸的痕迹。然而，纽蛋白在细菌周围和整个突起中均匀分布。感染志贺菌后，Henle细胞和HeLa细胞中的纽蛋白发生了深刻的重排：在未感染的细胞中，它主要分布在细胞周边，而在受感染的细胞中，它主要集中在细胞质中的细菌簇周围。这表明纽蛋白细胞骨架蛋白可能参与了感染期间志贺菌的细胞间传播。
