The direct excitatory effect of IL-1beta on cerebellar Purkinje cell.

Interleukin (IL)-1beta is one of the important proinflammatory cytokines in neural as well as immune systems, and plays a pivotal role in the neuroinflammation. We previously demonstrated that cerebellar IL-1beta is involved in kainate-induced ataxia, i.e., IL-1beta was activated in the cerebellum with systemic administration of kainate, and its type I receptor (IL-1R) was expressed at a soma of cerebellar Purkinje cells. In this study, we examined the effect of IL-1beta on cerebellar Purkinje cell function by recording extracellular neuronal activities in anesthetized mice. Systemic administration of kainate increased the firing rates of cerebellar Purkinje cells in normal mice but showed little effect in IL-1R-knockout (IL-1R-KO) mice. Moreover, microiontophoretic administration of IL-1beta to cerebellar Purkinje cells increased the firing rates promptly in response to IL-1beta. The present results demonstrate that IL-1 system exerts a direct modulatory effect on cerebellar Purkinje cells.