Kwon J, Park J, Lee D, Kim Y S, Jeong H J
Research Institute for Transplantation and Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.
Transplant Proc. 2008 Dec;40(10):3479-80. doi: 10.1016/j.transproceed.2008.06.073.
Toll-like receptor (TLR) is known to be a mediator of innate immunity, but recent reports have shown that TLR provides a link to adaptive immunity involved in allograft rejection. To explore the expression patterns in various conditions of renal transplantation, we examined TLR subunit mRNA expressions in renal allograft biopsies of acute rejection (AR; n = 11), chronic rejection (CR; n = 15), chronic cyclosporine nephrotoxicity (CsAN; n = 22), and immunoglobulin A nephropathy (IgAN; n = 9) patients. Control tissues (n = 7) were obtained from normal renal cortical tissue of renal cell carcinoma patients. The diagnosis was made according to the Banff 97 classification. The expressions of TLR 2, 3, 4, and 9 mRNA were analyzed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) using SYBR green. Statistical analysis was performed using analysis of variance (ANOVA) and the Student t test. TLR 2 and 3 mRNA expressions were not significantly different in any group (P > .05). In contrast, TLR 4 mRNA expression was significantly increased in all allograft groups compared with that of controls, and significantly higher in the CsAN than other transplant groups (P < .05). TLR 9 mRNA expression was up-regulated in CsAN and IgAN compared with AR and CR (P < .05). These results suggested that TLR4 mRNA expression was increased in renal allograft patients with chronic allograft dysfunction. Further studies are needed to correlate TLR subtypes with various causes of graft dysfunction among renal allograft patients.
Toll样受体（TLR）是已知的天然免疫介质，但最近的报告表明，TLR为参与同种异体移植排斥反应的适应性免疫提供了联系。为了探索肾移植各种情况下的表达模式，我们检测了急性排斥反应（AR；n = 11）、慢性排斥反应（CR；n = 15）、慢性环孢素肾毒性（CsAN；n = 22）和免疫球蛋白A肾病（IgAN；n = 9）患者肾移植活检中TLR亚基mRNA的表达。对照组织（n = 7）取自肾细胞癌患者的正常肾皮质组织。诊断根据Banff 97分类标准进行。使用SYBR green通过实时逆转录聚合酶链反应（RT-PCR）分析TLR 2、3、4和9 mRNA的表达。采用方差分析（ANOVA）和学生t检验进行统计分析。TLR 2和3 mRNA的表达在任何组中均无显著差异（P > 0.05）。相比之下，与对照组相比，所有同种异体移植组中TLR 4 mRNA的表达均显著增加，且CsAN组显著高于其他移植组（P < 0.05）。与AR和CR相比，CsAN和IgAN中TLR 9 mRNA的表达上调（P < 0.05）。这些结果表明，慢性同种异体移植功能障碍的肾移植患者中TLR4 mRNA表达增加。需要进一步研究肾移植患者中TLR亚型与移植功能障碍各种原因之间的相关性。
