Zozulińska M, Zawilska K
Kliniki Hematologii Instytutu Chorób Wewnetrznych Akademii Medycznej im Karola Marcinkowskiego w Poznaniu.
Pol Arch Med Wewn. 1991 May;85(5):286-93.
In 33 insulin-dependent, I and II type diabetic patients the authors evaluated the intraplatelet concentration of 12-hydroperoxyeicozatetraenoic acid (12-HPETE) and malonylodialdehyde (MDA) which are the products of lipoxygenase (LO) and cyclooxygenase (CO) metabolism of arachidonic acid (AA) in blood platelets. Moreover, in all patients, determinations of cholesterol total lipids, phospholipids, triacylglycerols were performed as well as serum lipoproteinogram. The studies were done in diabetic ketoacidosis and 2 weeks after compensation of diabetes was attained. Sixty healthy persons, with no changes in the coagulation system, constituted the control group. In patients with diabetic ketoacidosis a higher intraplatelet concentration of 12-HPETE (7.2 +/- 4.0 nmol/10(9) platelets) was found as compared with the values observed in the control group (4.7 +/- 2.1 nmol MDA/10(9) platelets); p less than 0.01. Intraplatelet MDA concentration did not, however, show a statistically significant difference. When compensation of diabetes was obtained the mean intraplatelet 12-HPETE concentration fell to values close the normal ones (5.5 +/- 3.4 nmol MDA/10(9) platelets). Nevertheless, the results of comparative determinations of mean values of both 12-HPETE and MDA concentrations in ketoacidosis as well as in compensated diabetes did not show statistically significant difference. High intraplatelet 12-HPETE concentration in diabetic ketoacidosis may be a cause of the formation or intensification of atherosclerotic changes, typical of this group of patients. The studies did not prove any correlation between the intraplatelet concentration of AA metabolism products and blood glucose concentration and lipid metabolism products. Neither was there any correlation between 12-HPETE and MDA concentration and the duration of clinically symptomatic diabetes.
作者对33例I型和II型胰岛素依赖型糖尿病患者血小板内12 - 氢过氧化二十碳四烯酸（12 - HPETE）和丙二醛（MDA）的浓度进行了评估，这两种物质是血小板中花生四烯酸（AA）经脂氧合酶（LO）和环氧化酶（CO）代谢的产物。此外，对所有患者还进行了总胆固醇、总脂质、磷脂、三酰甘油的测定以及血清脂蛋白谱分析。研究在糖尿病酮症酸中毒时以及糖尿病得到代偿2周后进行。60名凝血系统无异常的健康人作为对照组。糖尿病酮症酸中毒患者血小板内12 - HPETE浓度（7.2±4.0 nmol/10⁹血小板）高于对照组（4.7±2.1 nmol MDA/10⁹血小板）；p＜0.01。然而，血小板内MDA浓度未显示出统计学上的显著差异。糖尿病得到代偿后，血小板内12 - HPETE平均浓度降至接近正常的值（5.5±3.4 nmol MDA/10⁹血小板）。尽管如此，酮症酸中毒以及代偿性糖尿病时12 - HPETE和MDA浓度平均值的比较测定结果未显示出统计学上的显著差异。糖尿病酮症酸中毒时血小板内高浓度的12 - HPETE可能是该组患者典型的动脉粥样硬化改变形成或加重的原因。研究未证实AA代谢产物的血小板内浓度与血糖浓度及脂质代谢产物之间存在任何相关性。12 - HPETE和MDA浓度与临床症状性糖尿病的病程之间也无相关性。
