Gebauer Petr, Malá Zdena, Bocek Petr
Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
Electrophoresis. 2009 Jan;30(1):29-35. doi: 10.1002/elps.200800425.
ITP is an electrophoretic technique that has been attracting constant attention for many years due to its pronounced capability to concentrate trace analytes by several orders of magnitude. In practice, it is used predominantly in capillary format, where the capillaries used have id ranging between 0.02 and 0.8 mm. The volumes of the samples introduced may be up to several tens of microliters and trace analytes diluted in such a volume are concentrated into zones having volumes in the range of picoliters. Moreover, it offers simultaneously efficient separation of the analytes. That is why ITP retains its important position in many current multistage and multidimensional separation schemes where it is used always as the starting step that brings preseparation and concentration of sample components. This article links up previous reviews on the topic and summarizes the progress of analytical capillary ITP since 2006; 90 reviewed papers include theory and methodological novelties as well as analytical applications. Papers using ITP as part of multistage separation schemes are also included.
等速电泳（ITP）是一种电泳技术，多年来一直备受关注，因为它具有将痕量分析物浓缩几个数量级的显著能力。在实际应用中，它主要以毛细管形式使用，所用毛细管的内径范围在0.02至0.8毫米之间。引入的样品体积可达几十微升，稀释在该体积中的痕量分析物被浓缩到体积在皮升范围内的区域。此外，它还能同时对分析物进行高效分离。这就是为什么ITP在许多当前的多级和多维分离方案中仍保持重要地位，在这些方案中，它总是作为起始步骤，对样品组分进行预分离和浓缩。本文衔接了此前关于该主题的综述，并总结了自2006年以来分析型毛细管等速电泳的进展；90篇综述论文涵盖了理论和方法创新以及分析应用。还包括将ITP用作多级分离方案一部分的论文。
