Shi Ying, Wei Fei-Li, Liu Ya-Li, Ji Yun-Xia, Wu Hao, Chen De-Xi, Zhou Yu-Sen
Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008 Aug;22(4):287-9.
To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.
TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.
TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.
HBX protein could be induced into mouse liver by TAT induced peptide.
高效表达TAT-HBX-EGFP融合蛋白并研究其在小鼠肝脏中的分布。
构建TAT-HBX-EGFP重组载体，用异丙基-β-D-硫代半乳糖苷（IPTG）诱导融合蛋白在BL21中表达；用镍-亚氨基二乙酸琼脂糖凝胶（Ni-NTA agarose）纯化融合蛋白，然后注入小鼠腹腔。通过免疫荧光观察融合蛋白的分布。
TAT-HBX-EGFP在大肠杆菌中高效表达；HBX可通过TAT导入小鼠肝脏。
TAT诱导肽可将HBX蛋白导入小鼠肝脏。
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