人类造血细胞的基因改造：用于临床试验的逆转录病毒介导基因转移的临床前优化
Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials.
作者信息
Budak-Alpdogan Tulin, Rivière Isabelle
机构信息
Department of Medicine, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ, USA.
出版信息
Methods Mol Biol. 2009;506:33-58. doi: 10.1007/978-1-59745-409-4_4.
Abstract
This chapter provides information about the oncoretroviral transduction of human hematopoietic stem/ progenitor cells under clinically applicable conditions. We describe in detail a short -60 h transduction protocol which consistently yields transduction efficiencies in the range of 30-50% with five different oncoretroviral vectors. We discuss a number of parameters that affect transduction efficiency, including the oncoretroviral vector characteristics, the vector stock collection, the source of CD34+ cells and transduction conditions.
摘要
本章提供了在临床适用条件下人类造血干/祖细胞的嗜肝性逆转录病毒转导的相关信息。我们详细描述了一个为期60小时的简短转导方案，该方案使用五种不同的嗜肝性逆转录病毒载体，始终能产生30%-50%的转导效率。我们讨论了一些影响转导效率的参数，包括嗜肝性逆转录病毒载体特性、载体储备收集、CD34+细胞来源和转导条件。
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