Franceschi Loretta, Faggiani Anna, Furlanut Mario
Institute of Clinical Pharmacology and Toxicology, University of Udine, Udine, Italy.
J Pharm Biomed Anal. 2009 Feb 20;49(2):554-7. doi: 10.1016/j.jpba.2008.11.017. Epub 2008 Nov 27.
A rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with MS/MS detection for simultaneous quantification of fluoxetine and its major active metabolite in serum samples has been developed. Analytes were extracted with a simple three step liquid-liquid procedure and chromatographic separation was achieved on a C18 column. Because of its sensitivity, this HPLC/MS/MS method is suitable both for routine therapeutic drug monitoring and for pharmacokinetic studies, due to its low limits of quantification.
已开发出一种快速、选择性和灵敏的等度反相高效液相色谱法，结合串联质谱检测，用于同时定量血清样本中的氟西汀及其主要活性代谢物。分析物通过简单的三步液液萃取程序进行提取，并在C18柱上实现色谱分离。由于其灵敏度高，这种高效液相色谱/串联质谱方法因其低定量限，既适用于常规治疗药物监测，也适用于药代动力学研究。
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