Demirel Gökçen Birlik, Coşkun Samet, Kalkan Meryem, Caykara Tuncer
Department of Chemistry, Faculty of Arts and Science, Gazi University, Beşevler, Ankara, Turkey.
Macromol Biosci. 2009 May 13;9(5):472-9. doi: 10.1002/mabi.200800226.
The design of a novel polymer-modified overlayer composed of PPAPE and GPMS on a silicon wafer for immobilization of DNA molecules is described. After hydroxylation of Si(100) surfaces, GPMS molecules were self-assembled onto these surfaces. PPAPE molecules were then covalently attached to the epoxy-terminated surfaces. The incubation time and concentration of PPAPE was found to effect both layer thickness and water CA. The type of organic solvent and the pH were found to change the nature of the PPAPE-modified surface for DNA immobilization. It is concluded that PPAPE-modified surfaces show advantages for DNA immobilization by electrostatic interactions between DNA molecules and positively charged free amino groups of the PPAPE-modified surfaces at the appropriate pH values.
描述了一种在硅片上由PPAPE和GPMS组成的用于固定DNA分子的新型聚合物修饰覆盖层的设计。在Si(100)表面羟基化后，GPMS分子自组装到这些表面上。然后将PPAPE分子共价连接到环氧封端的表面。发现PPAPE的孵育时间和浓度会影响层厚度和水接触角。发现有机溶剂的类型和pH会改变用于DNA固定的PPAPE修饰表面的性质。得出的结论是，在适当的pH值下，PPAPE修饰的表面通过DNA分子与PPAPE修饰表面带正电的游离氨基之间的静电相互作用显示出固定DNA的优势。
