西罗莫司对膀胱癌细胞系T24的作用。
Effect of sirolimus on urinary bladder cancer T24 cell line.
作者信息
Pinto-Leite Rosario, Botelho Pedro, Ribeiro Eufemia, Oliveira Paula A, Santos Lucios
机构信息
Genetic Service, Cytogenetic laboratory, Hospital center of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
出版信息
J Exp Clin Cancer Res. 2009 Jan 7;28(1):3. doi: 10.1186/1756-9966-28-3.
BACKGROUND
Sirolimus is recently reported to have antitumour effects on a large variety of cancers. The present study was performed to investigate sirolimus's ability to inhibit growth in T24 bladder cancer cells.
METHODS
T24 bladder cancer cells were treated with various concentrations of sirolimus. MTT assay was used to evaluate the proliferation inhibitory effect on T24 cell line. The viability of T24 cell line was determined by Trypan blue exclusion analysis.
RESULTS
Sirolimus inhibits the growth of bladder carcinoma cells and decreases their viability. Significant correlations were found between cell proliferation and sirolimus concentration (r = 0.830; p < 0.01) as well as between cell viability and sirolimus concentration (r = -0.896; p < 0.01).
CONCLUSION
Sirolimus has an anti-proliferation effect on the T24 bladder carcinoma cell line. The information from our results is useful for a better understanding sirolimus's anti-proliferative activity in the T24 bladder cancer cell line.
背景
西罗莫司最近被报道对多种癌症具有抗肿瘤作用。本研究旨在探讨西罗莫司抑制T24膀胱癌细胞生长的能力。
方法
用不同浓度的西罗莫司处理T24膀胱癌细胞。采用MTT法评估对T24细胞系的增殖抑制作用。通过台盼蓝排斥分析测定T24细胞系的活力。
结果
西罗莫司抑制膀胱癌细胞的生长并降低其活力。发现细胞增殖与西罗莫司浓度之间存在显著相关性（r = 0.830；p < 0.01），细胞活力与西罗莫司浓度之间也存在显著相关性（r = -0.896；p < 0.01）。
结论
西罗莫司对T24膀胱癌细胞系具有抗增殖作用。我们的研究结果有助于更好地理解西罗莫司在T24膀胱癌细胞系中的抗增殖活性。
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