水稻条纹病毒的假定运动蛋白Pc4与水稻的一种I型DnaJ蛋白和一种小热激蛋白相互作用。
Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice.
作者信息
Lu Lianming, Du Zhenguo, Qin Meiling, Wang Ping, Lan Hanhong, Niu Xiaoqing, Jia Dongsheng, Xie Liyan, Lin Qiying, Xie Lianhui, Wu Zujian
机构信息
Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
出版信息
Virus Genes. 2009 Apr;38(2):320-7. doi: 10.1007/s11262-008-0324-z. Epub 2009 Jan 8.
Rice stripe virus (RSV) infects rice and causes great yield reduction in some Asian countries. In this study, rice cDNA library was screened by a Gal4-based yeast two-hybrid system using pc4, a putative movement protein of RSV, as the bait. A number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. Two of them were selected and further characterized. The two proteins were a J protein and a small Hsp, respectively. Interactions between Pc4 and the two proteins were confirmed using coimmunoprecipitation. Implications of the findings that pc4 interacted with two chaperone proteins were discussed.
水稻条纹病毒（RSV）感染水稻，并在一些亚洲国家导致大幅减产。在本研究中，以RSV的一种假定运动蛋白Pc4为诱饵，通过基于Gal4的酵母双杂交系统筛选水稻cDNA文库。鉴定出许多阳性菌落，序列分析表明它们可能对应十种独立的蛋白质。从中选择了两种并进行进一步表征。这两种蛋白质分别是一种J蛋白和一种小热激蛋白。通过免疫共沉淀证实了Pc4与这两种蛋白质之间的相互作用。讨论了Pc4与两种伴侣蛋白相互作用这一发现的意义。
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