鼻窦癌的微卫星不稳定性分析
Microsatellite instability analysis of sinonasal carcinomas.
作者信息
Martínez Jorge García, Pérez-Escuredo Jhudit, López Fernando, Suárez Carlos, Alvarez-Marcos César, Llorente José Luis, Hermsen Mario A
机构信息
Department of Otorhinolaryngology, IUOPA, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain.
出版信息
Otolaryngol Head Neck Surg. 2009 Jan;140(1):55-60. doi: 10.1016/j.otohns.2008.10.038.
OBJECTIVES
Intestinal-type sinonasal adenocarcinoma (ITAC) and squamous cell carcinoma of the nasal cavity (SCCNC) are histopathologically but not etiologically similar to colorectal adenocarcinoma or to laryngeal squamous cell carcinoma, respectively. Microsatellite instability (MSI) is involved in both tumors. The aim of this study was to investigate a possible role for MSI in the pathogenesis of two types of nasal carcinoma.
MATERIAL AND METHODS
DNA obtained from frozen tumor samples of 41 ITACs and 24 SCCNCs was analyzed for shifts in five mononucleotide microsatellite loci by multiplex PCR.
RESULTS
The allelic patterns of one ITAC (2%) and five SCCNCs (21%) revealed an allelic shift for at least one of the five loci, indicating microsatellite instability.
CONCLUSION
MSI may be involved in squamous cell carcinoma, but not in adenocarcinoma of the nasal cavities.
目的
肠型鼻窦腺癌（ITAC）和鼻腔鳞状细胞癌（SCCNC）在组织病理学上分别与结直肠腺癌或喉鳞状细胞癌相似，但病因不同。微卫星不稳定性（MSI）在这两种肿瘤中均有涉及。本研究的目的是探讨MSI在两种鼻腔癌发病机制中的可能作用。
材料与方法
通过多重PCR分析从41例ITAC和24例SCCNC的冷冻肿瘤样本中获得的DNA在五个单核苷酸微卫星位点的变化。
结果
1例ITAC（2%）和5例SCCNC（21%）的等位基因模式显示，五个位点中至少有一个出现等位基因移位，表明存在微卫星不稳定性。
结论
MSI可能参与鼻腔鳞状细胞癌的发生，但不参与鼻腔腺癌的发生。
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