糖尿病患者单核细胞αL、αM和β2整合素亚基增加。
Increased monocyte alphaL, alphaM and beta2 integrin subunits in diabetes mellitus.
作者信息
Kostidou Elena, Koliakos George, Kaloyianni Martha
机构信息
Department of Zoology, Laboratory of Animal Physiology, School of Biology, Aristotle University, Thessaloniki, Greece.
出版信息
Clin Biochem. 2009 May;42(7-8):634-40. doi: 10.1016/j.clinbiochem.2008.12.007. Epub 2008 Dec 24.
OBJECTIVES
Previous studies indicated the involvement of alpha2 (CD49b) integrin subunit in monocyte-laminin attachment in diabetes. The aim of the present study was to further investigate monocyte atherosclerosis related integrin subunit changes in relation to diabetes and hyperglycemia.
DESIGN AND METHODS
Monocytes were derived from 10 patients with type II diabetes and 10 age matched controls. Attachment to laminin-1 in the presence of anti-integrin subunit antibodies has been estimated by a solid phase assay while for the alpha2 integrin subunit density assessment, fluorescence spectrometry was used.
RESULTS
Incubation of monocytes with monoclonal anti-alphaL (CD11a) and anti-beta2 (CD18) equalized the differences between diabetics and controls. Both glucose and insulin increased the alpha2 integrin subunit expression in relation to the controls.
CONCLUSIONS
The previously observed modified interaction between monocytes and laminin in diabetes mellitus may be attributed to changes not only in alpha2 but also in alphaL and beta2 integrin subunit changes.
目的
先前的研究表明α2（CD49b）整合素亚基参与糖尿病患者单核细胞与层粘连蛋白的黏附。本研究的目的是进一步研究与糖尿病和高血糖相关的单核细胞动脉粥样硬化相关整合素亚基的变化。
设计与方法
单核细胞来自10例II型糖尿病患者和10例年龄匹配的对照者。通过固相分析评估在抗整合素亚基抗体存在下与层粘连蛋白-1的黏附情况，而对于α2整合素亚基密度评估，则使用荧光光谱法。
结果
用单克隆抗αL（CD11a）和抗β2（CD18）孵育单核细胞可消除糖尿病患者和对照者之间的差异。与对照相比，葡萄糖和胰岛素均增加了α2整合素亚基的表达。
结论
先前观察到的糖尿病患者单核细胞与层粘连蛋白之间的相互作用改变，可能不仅归因于α2，还归因于αL和β2整合素亚基的变化。
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