Mandal Santi M, Mandal Mahitosh, Das Amit K, Pati Bikas R, Ghosh Ananta K
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
Arch Microbiol. 2009 Apr;191(4):389-93. doi: 10.1007/s00203-008-0455-6. Epub 2009 Jan 17.
The influence of endogenous root nodules phenolic acids on indoleacetic acid (IAA) production by its symbiont (Rhizobium) was examined. The root nodules contain higher amount of IAA and phenolic acids than non-nodulated roots. Presence of IAA metabolizing enzymes, IAA oxidase, peroxidase, and polyphenol oxidase indicate the metabolism of IAA in the nodules and roots. Three most abundant endogenous root nodule phenolic acids (protocatechuic acid, 4-hydroxybenzaldehyde and p-coumaric acid) have been identified and their effects on IAA production by the symbiont have been studied in L-tryptophan supplemented yeast extract basal medium. Protocatechuic acid (1.5 microg ml(-1)) showed maximum stimulation (2.15-fold over control) of IAA production in rhizobial culture. These results indicate that the phenolic acids present in the nodule might serve as a stimulator for IAA production by the symbiont (Rhizobium).
研究了内生根瘤酚酸对其共生体（根瘤菌）产生吲哚乙酸（IAA）的影响。根瘤中IAA和酚酸的含量高于未结瘤的根。IAA代谢酶、IAA氧化酶、过氧化物酶和多酚氧化酶的存在表明IAA在根瘤和根中发生了代谢。已鉴定出三种最丰富的内生根瘤酚酸（原儿茶酸、4-羟基苯甲醛和对香豆酸），并在添加L-色氨酸的酵母提取物基础培养基中研究了它们对共生体产生IAA的影响。原儿茶酸（1.5微克/毫升）在根瘤菌培养中对IAA产生表现出最大刺激作用（比对照高2.15倍）。这些结果表明，根瘤中存在的酚酸可能作为共生体（根瘤菌）产生IAA的刺激物。
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