通过诱变和荧光结合分析对细胞视黄酸结合蛋白 II 的关键结合决定簇进行剖析。
Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.
机构信息
Department of Chemistry, Michigan State University, East Lansing, 48824, USA.
出版信息
Proteins. 2009 Aug 1;76(2):281-90. doi: 10.1002/prot.22334.
Abstract
The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue.
摘要
评估维甲酸与细胞维甲酸结合蛋白 II (CRABPII) 突变体的结合情况，以更好地了解直接的蛋白质/配体相互作用的重要性。验证了 Arg111 对蛋白质正确结构和功能的重要作用，并确定了其他直接影响维甲酸结合的残基。此外，通过提供 Glu 残基形式的羧酸二聚体伙伴，挽救了缺乏所有先前鉴定的相互作用氨基酸的 CRABPII 突变体与维甲酸的结合。
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