快速合成多组分肽混合物的通用方法。

General method for rapid synthesis of multicomponent peptide mixtures.

作者信息

Furka A, Sebestyén F, Asgedom M, Dibó G

机构信息

Department of Organic Chemistry, Eötvös Loránd University, Budapest, Hungary.

出版信息

Int J Pept Protein Res. 1991 Jun;37(6):487-93. doi: 10.1111/j.1399-3011.1991.tb00765.x.

DOI:10.1111/j.1399-3011.1991.tb00765.x

PMID:1917305

Abstract

A method is suggested for the synthesis of multicomponent peptide mixtures. The method is a solid phase synthesis modified in order to give a closely equimolar mixture of peptides with predetermined sequences. The main point of modification is that before every coupling cycle the resin is divided into equal parts and each portion is coupled with a different amino acid. Then the portion are mixed and before the next coupling cycle the resin is again distributed into equal portions. The method is illustrated by the synthesis of a mixture of 27 tetrapeptides and that of 180 pentapeptides.

摘要

提出了一种合成多组分肽混合物的方法。该方法是一种经过改进的固相合成法，旨在得到具有预定序列的紧密等摩尔肽混合物。改进的要点在于，在每个偶联循环之前，将树脂分成相等的部分，每一部分与不同的氨基酸进行偶联。然后将这些部分混合，在下一个偶联循环之前，再次将树脂分成相等的部分。通过合成27种四肽的混合物和180种五肽的混合物对该方法进行了说明。