[二肽基肽酶IV（DPPIV）表达与上皮性卵巢癌临床病理特征及预后的相关性]

[Correlation of DPPIV expression with clinicopathological features and prognosis in epithelial ovarian carcinoma].

作者信息

Zhang Meng-zhen, Qiao Yu-huan, Suo Zhen-he

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2008 Nov;30(11):848-52.

DOI:

PMID:19173831

Abstract

OBJECTIVE

To investigate the expression of dipeptidyl peptidase IV (DPPIV) in patients with epithelial ovarian carcinoma (EOC) and its clinical significance.

METHODS

Immunohistochemistry (IHC) was used to detect the expression of DPPIV protein in 378 formalin-fixed paraffin-embedded EOC tissue samples. The expression of DPPIV mRNA in 86 EOC tissue samples were examined by in situ hybridization (ISH) using specific FITC-labelled RNA probes. Forty-two samples of normal ovarian tissues were used as control. Statistical analyses were carried out by Chi-square test, Spearman rank correlation and Kaplan-Meier method.

RESULTS

Among the 378 epithelial ovarian carcinomas, 351 (92.9%) showed a positive expression of DPPIV protein, while only 25/42 (59.5%) of normal ovaries had a positive expression by semi-quantitative IHC analysis. The expression level of DPPIV protein was significantly lower in the normal ovaries than that in ovarian carcinomas (chi(2) = 18.4, P = 0.001). There was no significant correlation between the expression of DPPIV protein and age, FIGO stage and histological grade (P > 0.05). However, the expression of DPPIV protein was significantly associated with histological type (chi(2) = 28.5, P = 0.005). The patients with high level expression of DPPIV protein likely had a poor prognosis in terms of overall survival (P = 0.02). Of the 86 patients, 84 (97.7%)showed positive expression of DPPIV mRNA, also higher than that in normal ovarian tissues (P < 0.05). A statistically significant correlation between DPPIV mRNA and protein expression was observed (r(s) = 0.66, P = 0.001).

CONCLUSION

DPPIV may be involved in the carcinogenesis of ovarian cancer, and may become a potential prognostic marker for epithelial ovarian carcinoma.

摘要

目的

探讨二肽基肽酶IV（DPPIV）在上皮性卵巢癌（EOC）患者中的表达及其临床意义。

方法

采用免疫组织化学（IHC）法检测378例福尔马林固定石蜡包埋的EOC组织样本中DPPIV蛋白的表达。使用特异性异硫氰酸荧光素（FITC）标记的RNA探针，通过原位杂交（ISH）检测86例EOC组织样本中DPPIV mRNA的表达。42例正常卵巢组织样本作为对照。采用卡方检验、Spearman等级相关分析和Kaplan-Meier法进行统计学分析。

结果

在378例上皮性卵巢癌中，351例（92.9%）DPPIV蛋白呈阳性表达，而通过半定量免疫组织化学分析，正常卵巢组织中仅25/42例（59.5%）呈阳性表达。正常卵巢组织中DPPIV蛋白的表达水平显著低于卵巢癌组织（χ2 = 18.4，P = 0.001）。DPPIV蛋白的表达与年龄、国际妇产科联盟（FIGO）分期及组织学分级之间无显著相关性（P > 0.05）。然而，DPPIV蛋白的表达与组织学类型显著相关（χ2 = 28.5，P = 0.005）。DPPIV蛋白高表达的患者总体生存预后可能较差（P = 0.）。在86例患者中，84例（97.7%）DPPIV mRNA呈阳性表达，也高于正常卵巢组织（P < 0.05）。观察到DPPIV mRNA与蛋白表达之间存在统计学显著相关性（rs = 0.66，P = 0.001）。