[血管加压素对大鼠内耳水通道蛋白7表达的影响]
[Effect of vasopressin on aquaporin 7 expression in rat inner ear].
作者信息
Gu Feng-Ming, Zhang Lian-Shan
机构信息
Department of Otolaryngology, EYE and ENT Hospital, Fudan University, Shanghai 200031, China.
出版信息
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2008 Dec;30(6):659-62.
OBJECTIVE
To study the effect of vasopressin on aquaporin 7 (AQP7) expression in rat inner ear and reveal the possible role of aquaporins in the formation of endolymphatic hydrops induced by vasopressin.
METHODS
Wistar rats were intraperitoneally injected with 50 microg/kg arginine vasopressin once a day for one week. Differentially expressed genes of aquaporins induced by vasopressin injection in rat inner ear were filtered by cDNA microarray. The changes of mRNA expression level of AQP7 in inner ear of rats treated with vasopressin injection were measured by RT-PCR.
RESULTS
Differentially expressed gene AQP7 of aquaporins induced by vasopressin injection was screened out in rat inner ear. The expression level of AQP7 mRNA in inner ear of rats treated with vasopressin injection was significantly lower.
CONCLUSION
Vasopressin may down-regulate the expression of AQP7 mRNA in the endolymphatic sac and induce a decreased absorption of endolymph, which decreases the water permeability in the potassium ions recycle pathway in the organ of Corti and disturbs the circulation of endolymph, resulting in endolymphatic hydrops.
目的
研究血管加压素对大鼠内耳水通道蛋白7（AQP7）表达的影响，揭示水通道蛋白在血管加压素诱导的内淋巴积水形成中的可能作用。
方法
Wistar大鼠每天腹腔注射50μg/kg精氨酸血管加压素，连续一周。通过cDNA微阵列筛选血管加压素注射诱导的大鼠内耳水通道蛋白差异表达基因。采用RT-PCR检测血管加压素注射处理大鼠内耳AQP7 mRNA表达水平的变化。
结果
在大鼠内耳筛选出血管加压素注射诱导的水通道蛋白差异表达基因AQP7。血管加压素注射处理大鼠内耳AQP7 mRNA表达水平显著降低。
结论
血管加压素可能下调内淋巴囊AQP7 mRNA的表达，导致内淋巴吸收减少，降低柯蒂器钾离子循环途径的水通透性，扰乱内淋巴循环，从而导致内淋巴积水。
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