Schultz R M, Wassarman P M
Proc Natl Acad Sci U S A. 1977 Feb;74(2):538-41. doi: 10.1073/pnas.74.2.538.
High resolution two-dimensional electrophoresis has been used to examine the pattern of protein synthesis during meiotic maturation of mouse oocytes in vitro. Fluorograms of [35S]methionine-labeled oocyte proteins have revealed that meiotic progression from dictyate to metaphase II (meiotic maturation) is accompanied by marked changes in the pattern of proteins synthesized by oocytes. Virtually all of the changes observed take place subsequent to the breakdown of the oocyte's germinal vesicle, but are not dependent upon the occurrence of other morphological events, such as spindle formation or polar body emission. These changes in protein synthesis do not take place in oocytes that fail to undergo breakdown of germinal vesicles spontaneously or in oocytes arrested at the germinal vesicle stage by dibutyryl 3':5'-cyclic AMP. These data suggest that mixing of the oocyte's nucleoplasm and cytoplasm may trigger many of the changes in protein synthesis that accompany meiotic maturation of mouse oocytes in vitro.
高分辨率二维电泳已被用于检测体外培养的小鼠卵母细胞减数分裂成熟过程中的蛋白质合成模式。[35S]甲硫氨酸标记的卵母细胞蛋白质荧光图谱显示，从双线期到中期II的减数分裂进程（减数分裂成熟）伴随着卵母细胞合成蛋白质模式的显著变化。几乎所有观察到的变化都发生在卵母细胞生发泡破裂之后，但并不依赖于其他形态学事件的发生，如纺锤体形成或极体排放。在未能自发经历生发泡破裂的卵母细胞或被二丁酰3':5'-环磷酸腺苷阻滞在生发泡期的卵母细胞中，蛋白质合成没有发生这些变化。这些数据表明，卵母细胞核质与细胞质的混合可能触发了体外培养的小鼠卵母细胞减数分裂成熟过程中蛋白质合成的许多变化。
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