空肠弯曲菌大缺失突变体的构建表明脂寡糖基因簇对生存力并非必需。
Creation of a large deletion mutant of Campylobacter jejuni reveals that the lipooligosaccharide gene cluster is not required for viability.
作者信息
Marsden Gemma L, Li Jianjun, Everest Paul H, Lawson Andrew J, Ketley Julian M
机构信息
Department of Genetics, University of Leicester, United Kingdom.
出版信息
J Bacteriol. 2009 Apr;191(7):2392-9. doi: 10.1128/JB.01397-08. Epub 2009 Jan 30.
Abstract
Deletion of the lipooligosaccharide biosynthesis region (Cj1132c to Cj1152c) from the genome of Campylobacter jejuni NCTC11168 shows that the core is not required for viability. The mutant was attenuated for growth and has increased sensitivity to antibiotics and detergents. Natural transformation and invasion of cultured host cells was abolished.
摘要
从空肠弯曲菌NCTC11168基因组中删除脂寡糖生物合成区域（Cj1132c至Cj1152c）表明，其核心部分并非生存所必需。该突变体生长减弱，对抗生素和去污剂的敏感性增加。其对培养宿主细胞的自然转化和侵袭能力丧失。
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