应用抗原竞争 ELISA 检测实验感染鸡传染性支气管炎病毒。

Detection of infectious bronchitis virus in experimentally infected chickens by an antigen-competitive ELISA.

机构信息

Universidade Estadual Paulista, Faculdade de Cieinsertion markncias Agrarias e Veterinarias, Departmento de Patologia Veterinaria - Laboratorio de Imunologia e Virologia, Rodovia Prof. Paulo Donatto Castellani, Km 05, 14870- 000, Jaboticabal, SP, Brazil.

出版信息

Avian Pathol. 2001 Feb;30(1):67-71. doi: 10.1080/03079450020023221.

DOI:10.1080/03079450020023221

PMID:19184876

Abstract

An antigen-competitive enzyme-linked immunosorbent assay (Ag-C-ELISA) was developed for the detection of infectious bronchitis virus (IBV) antigens, M41 strain, in tissues from experimentally infected chickens, or in allantoic fluid harvested from inoculated embryonated eggs. The detection limit of IBV in the Ag-C-ELISA was 10(4.1) median embryo infective doses (EID(50))/well. Tracheal and lung samples from chickens vaccinated with 10(2.5) EID(50) of live attenuated infectious bronchitis (H120) vaccine were negative in the direct detection Ag-C-ELISA. The results indicate that the Ag-C-ELISA has the potential to detect IBV, either directly in tissue samples or when combined with the passage of material in embryonated eggs, thereby constituting an alternative method for the diagnosis of IBV.

摘要

建立了一种用于检测传染性支气管炎病毒（IBV）M41 株抗原的抗原竞争酶联免疫吸附试验（Ag-C-ELISA），可用于检测实验感染鸡的组织或接种鸡胚收获的羊水。Ag-C-ELISA 检测 IBV 的检测限为 10(4.1)个半数胚胎感染剂量（EID(50))/孔。用 10(2.5)个活减毒传染性支气管炎（H120）疫苗的 EID(50)接种的鸡的气管和肺组织在直接检测 Ag-C-ELISA 中为阴性。结果表明，Ag-C-ELISA 具有检测 IBV 的潜力，无论是直接检测组织样本还是与鸡胚材料的传代相结合，因此构成了 IBV 诊断的替代方法。