人参皂苷在亨廷顿舞蹈症的细胞模型中对纹状体神经元具有保护作用。
Ginsenosides protect striatal neurons in a cellular model of Huntington's disease.
作者信息
Wu Jun, Jeong Hye Kyoung, Bulin Sarah Elizabeth, Kwon Sung Won, Park Jeong Hill, Bezprozvanny Ilya
机构信息
Department of Physiology, UT Southwestern Medical Center at Dallas, Dallas, Texas 75390, USA.
出版信息
J Neurosci Res. 2009 Jun;87(8):1904-12. doi: 10.1002/jnr.22017.
Abstract
Ginseng, the root of Panax ginseng C.A. Meyer (Araliaceae), is a widely used herbal medicine. Ginsenosides, the active ingredients of ginseng, are the main components responsible for many beneficial actions of ginseng. In the present study, we tested 10 different ginsenosides in the previously developed in vitro Huntington's disease (HD) assay with primary medium spiny striatal neuronal cultures (MSN) from the YAC128 HD mouse model. We found that nanomolar concentrations of ginsenoside Rb1 and Rc effectively protected YAC128 medium spiny neurons from glutamate-induced apoptosis and that Rg5 was protective at micromolar concentration. The other seven ginsenosides tested were not effective or exerted toxic effects in MSN cultures. From further experiments, we suggested that neuroprotective effects of ginsenosides Rb1, Rc, and Rg5 could correlate with their ability to inhibit glutamate-induced Ca(2+) responses in cultured MSN. From these results we concluded that ginsenosides Rb1, Rc, and Rg5 offer a potential therapeutic choice for the treatment of HD and possibly other neurodegenerative disorders.
摘要
人参，五加科人参（Panax ginseng C.A. Meyer）的根，是一种广泛使用的草药。人参皂苷作为人参的活性成分，是许多人参有益作用的主要成分。在本研究中，我们使用来自YAC128亨廷顿舞蹈病（HD）小鼠模型的原代中型多棘纹状体神经元培养物（MSN），在先前开发的体外HD测定中测试了10种不同的人参皂苷。我们发现纳摩尔浓度的人参皂苷Rb1和Rc有效地保护YAC128中型多棘神经元免受谷氨酸诱导的凋亡，并且Rg5在微摩尔浓度下具有保护作用。测试的其他七种人参皂苷在MSN培养物中无效或产生毒性作用。通过进一步的实验，我们表明人参皂苷Rb1、Rc和Rg5的神经保护作用可能与其抑制培养的MSN中谷氨酸诱导的Ca(2+)反应的能力相关。从这些结果我们得出结论，人参皂苷Rb1、Rc和Rg5为HD以及可能的其他神经退行性疾病的治疗提供了一种潜在的治疗选择。
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本文引用的文献
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Full length mutant huntingtin is required for altered Ca2+ signaling and apoptosis of striatal neurons in the YAC mouse model of Huntington's disease.在亨廷顿舞蹈症的YAC小鼠模型中，全长突变型亨廷顿蛋白对于纹状体神经元Ca2+信号改变和细胞凋亡是必需的。
Neurobiol Dis. 2008 Jul;31(1):80-8. doi: 10.1016/j.nbd.2008.03.010. Epub 2008 Apr 16.
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Ginsenosides: are any of them candidates for drugs acting on the central nervous system?人参皂苷：它们中有任何一种是作用于中枢神经系统的药物候选物吗？
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Ginsenoside Rg3 inhibits human Kv1.4 channel currents by interacting with the Lys531 residue.人参皂苷Rg3通过与Lys531残基相互作用抑制人Kv1.4通道电流。
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Pathological cell-cell interactions are necessary for striatal pathogenesis in a conditional mouse model of Huntington's disease.在亨廷顿舞蹈病的条件性小鼠模型中，病理性细胞间相互作用对纹状体发病机制而言是必要的。
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The corticostriatal pathway in Huntington's disease.亨廷顿舞蹈病中的皮质纹状体通路。
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Evaluation of clinically relevant glutamate pathway inhibitors in in vitro model of Huntington's disease.在亨廷顿舞蹈病体外模型中对临床相关谷氨酸途径抑制剂的评估。
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20(S)-ginsenoside Rh2, a newly identified active ingredient of ginseng, inhibits NMDA receptors in cultured rat hippocampal neurons.20（S）-人参皂苷Rh2是一种新发现的人参活性成分，可抑制培养的大鼠海马神经元中的NMDA受体。
Eur J Pharmacol. 2006 Apr 24;536(1-2):69-77. doi: 10.1016/j.ejphar.2006.02.038. Epub 2006 Feb 28.
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Striatal neuronal apoptosis is preferentially enhanced by NMDA receptor activation in YAC transgenic mouse model of Huntington disease.在亨廷顿病的YAC转基因小鼠模型中，纹状体神经元凋亡优先通过NMDA受体激活而增强。
Neurobiol Dis. 2006 Feb;21(2):392-403. doi: 10.1016/j.nbd.2005.08.001. Epub 2005 Sep 13.
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Ginseng and diabetes.人参与糖尿病。
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