Nezis Ioannis P, Papassideri Issidora
Faculty of Biology, Department of Cell Biology and Biophysics, University of Athens, Athens, Greece.
Methods Enzymol. 2008;451:669-83. doi: 10.1016/S0076-6879(08)03237-0.
Oogenesis is a fundamental physiological process in insects. Successful oogenesis is critical for evolutionary success by transferring genetic information to the next generation. This is achieved by the normal maturation of the egg chamber (egg), which is accomplished through cell death of the cells that accompany the oocyte. Recent studies demonstrate that autophagy contributes to this cell death process. Hence, comprehension of the mechanisms that implicates autophagy during cell death in insect eggs is very important. Herein, we describe some experimental approaches that can be used to monitor autophagy in insect eggs.
卵子发生是昆虫的一个基本生理过程。成功的卵子发生对于通过将遗传信息传递给下一代来实现进化成功至关重要。这是通过卵室（卵子）的正常成熟来实现的，而卵室的成熟是通过伴随卵母细胞的细胞死亡来完成的。最近的研究表明，自噬有助于这一细胞死亡过程。因此，了解昆虫卵子细胞死亡过程中涉及自噬的机制非常重要。在此，我们描述了一些可用于监测昆虫卵子自噬的实验方法。
