Rodger Alison
University of Warwick, Coventry CV4 7AL, UK.
Sci Prog. 2008;91(Pt 4):377-96. doi: 10.3184/003685008X395517.
The technique of linear dichroism (LD) is a simple absorbance technique that uses two polarised light beams. Since only oriented molecules show different absorbances for different polarisations, LD detects only oriented molecules. In aqueous solutions, flow orientation is an attractive orientation methodology as it selects long molecules or molecular assemblies. LD thus is selective for molecules that are particularly challenging to study by more standard biophysical techniques. In this article, a brief review of the application of LD to DNA, DNA-drug systems, DNA-protein enzymatic complexes, fibrous proteins and membrane peptides and proteins is given.
线性二色性（LD）技术是一种使用两束偏振光的简单吸光技术。由于只有取向分子对不同偏振表现出不同的吸光度，所以LD仅检测取向分子。在水溶液中，流动取向是一种有吸引力的取向方法，因为它能选择长分子或分子聚集体。因此，LD对那些用更标准的生物物理技术研究起来特别具有挑战性的分子具有选择性。本文简要综述了LD在DNA、DNA-药物体系、DNA-蛋白质酶复合物、纤维状蛋白质以及膜肽和膜蛋白中的应用。
