深龋下方发炎的人类牙髓中的成熟树突状细胞。
Mature dendritic cells in inflamed human pulps beneath deep caries.
作者信息
Harmon Melissa A, Tew John G, Best Al M, Hahn Chin-Lo
机构信息
Department of Endodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA, USA.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 May;107(5):727-32. doi: 10.1016/j.tripleo.2008.11.020. Epub 2009 Feb 6.
OBJECTIVE
Dendritic cells (DCs) in pulps have been identified with markers for immature DCs and the relationship of DC maturity to pulpal health has not been carefully examined. We sought to test the hypothesis that the frequency of CD83+ mature DCs would correlate with caries invasion.
STUDY DESIGN
Pulps were collected from extracted teeth exhibiting (I) no caries (n = 9), (II) shallow dentinal caries (n = 5), and (III) deep caries (n = 9). Immature DCs (CD209+), mature DCs (CD83+), and monocytes/macrophages (CD14+) in three groups were enumerated immunohistochemically.
RESULTS
Mature DCs were frequently found beneath deep caries (21.3 mature DCs/3 grids versus <1 in groups I and II) with increasing numbers of CD209+ DCs and CD14+ cells. Co-localization of CD4+ T cells with mature DCs and macrophages was observed in deep caries.
CONCLUSION
Mature DCs were frequently found only beneath deep caries and these DCs were co-localized with CD4+ T cells suggesting antigen presentation.
目的
牙髓中的树突状细胞（DCs）已通过未成熟DCs的标志物得以鉴定，而DC成熟度与牙髓健康之间的关系尚未得到仔细研究。我们试图验证这一假设，即CD83+成熟DCs的频率与龋齿侵袭相关。
研究设计
从拔除的牙齿收集牙髓，这些牙齿表现为（I）无龋齿（n = 9）、（II）浅龋（n = 5）和（III）深龋（n = 9）。通过免疫组织化学方法对三组中的未成熟DCs（CD209+）、成熟DCs（CD83+）和单核细胞/巨噬细胞（CD14+）进行计数。
结果
在深龋下方经常发现成熟DCs（21.3个成熟DCs/3个视野，而I组和II组中<1个），同时CD209+ DCs和CD14+细胞数量增加。在深龋中观察到CD4+ T细胞与成熟DCs和巨噬细胞共定位。
结论
仅在深龋下方经常发现成熟DCs，并且这些DCs与CD4+ T细胞共定位，提示存在抗原呈递。
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