Guzmán-Martínez Nayelli, Silva-Herzog Flores Daniel, Méndez González Verónica, Martín-Pérez Silvia, Cerda-Cristerna Bernardino Isaac, Cohenca Nestor
Master in Endodontics Program, Faculty of Dentistry, University of San Luis Potosí, San Luis potosí, México.
Dent Traumatol. 2009 Feb;25(1):43-50. doi: 10.1111/j.1600-9657.2008.00741.x.
Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain and 24% ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain; group 2, Emdogain + EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain alone or in combination with 24% EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth.
关于牙釉质基质衍生物的再生作用以及再植牙中牙周膜去除的必要性，目前仍存在争议。本研究的目的是评估釉基质蛋白（Emdogain）和24%乙二胺四乙酸（EDTA）进行牙根处理对再植犬牙牙周愈合的影响。牙齿被拔除，进行根管治疗并按以下方式预处理：第1组，使用釉基质蛋白；第2组，使用釉基质蛋白+EDTA；第3组，使用EDTA。牙齿在口外放置30分钟后再植，用夹板固定15天，并在观察8周后处死动物。使用苏木精/伊红染色和马松三色染色进行组织学评估，并根据先前报道的组织学评估标准对结果进行评分。除阴性对照组外，所有组均在组织学上诊断出替代性牙根吸收。参数分析显示实验组之间无统计学显著差异。单独使用釉基质蛋白或与24%EDTA联合进行牙根预处理均未显示出胶原纤维再生的迹象，因此无法防止再植犬牙发生替代性牙根吸收。
