[从离体灌注的人脐静脉释放内皮素。I：离子霉素的作用]

[Release of endothelin from isolated perfused human umbilical vein. I: Effect of ionomycin].

作者信息

Haga H, Mizuno K, Hashimoto S, Tani M, Niimura S, Katoh K, Tanaka K, Saitoh I, Yamada Z, Fukuchi S

机构信息

Third Department of Internal Medicine, Fukushima Medical College, Japan.

出版信息

Nihon Jinzo Gakkai Shi. 1991 Jun;33(6):545-8.

PMID:1920933

Abstract

Endothelin-like immunoreactivity (ET-LI) was directly measured in the perfusate from the isolated human umbilical vein perfused with Krebs-Ringer solution. The identity of the immunoreactive peptide was confirmed as ET-1 by high-performance liquid chromatography. The rate of release of ET-LI was 86.7 +/- 25.9 (SE) fmol during the first perfusion period of 30 min, and it remained stable at least for 4 hours. Calcium ionophore ionomycin, added to the perfusion medium (10(-7)-10(-6) M), stimulated the ET-LI release in a dose-dependent fashion; it increased the rate of release by 29.1% and 143.4% over the control at the concentrations of 10(-7) and 10(-6) M, respectively. These results taken together with previous observations of synthesis of ET in cultured vascular endothelium provide direct evidence for local generation and subsequent release of ET from vascular beds of human beings.

摘要

在内皮素样免疫反应性（ET-LI）的测定中，我们直接检测了用 Krebs-Ringer 溶液灌注的离体人脐静脉灌流液中的 ET-LI。通过高效液相色谱法确认了免疫反应性肽的身份为 ET-1。在最初 30 分钟的灌注期内，ET-LI 的释放速率为 86.7±25.9（SE）fmol，并且至少在 4 小时内保持稳定。添加到灌注培养基（10⁻⁷ - 10⁻⁶ M）中的钙离子载体离子霉素以剂量依赖方式刺激 ET-LI 的释放；在 10⁻⁷ 和 10⁻⁶ M 浓度下，它分别比对照增加了 29.1%和 143.4%的释放速率。这些结果与先前在培养的血管内皮中 ET 合成的观察结果相结合，为人类血管床中 ET 的局部生成和随后释放提供了直接证据。