Shoji R, Yajima R, Yano Y
Department of Chemical Science and Engineering, Tokyo National College of Technology, 1220-2 Kunugida, Hachioji, Tokyo 193-0997, Japan.
J Environ Sci (China). 2008;20(12):1463-8. doi: 10.1016/s1001-0742(08)62550-4.
Arsenic (As) speciation for the phytoremediation by the Chinese brake fern was studied. In particular, the mechanism of how plants induce compounds containing thiol (SH) and proteins by As exposure in terms of the relationship between As and phosphate uptaken into plant cells was examined. Pteris vittata callus could efficiently reduce As(V) to As(III) by the rapid introduction of reductase and synthesize thiols leading to phytochelatins production. Furthermore, Pteris vittata could control phosphate concentration in the cells corresponding to the concentration of arsenite and arsenate. To our best knowledge, this is the first report to show the mechanisms of such high As tolerance of Pteris vittata using their callus in terms of in vitro approach for the analysis of As speciation and metabolism route.
研究了蜈蚣草对砷的植物修复中的砷形态。特别地，从植物细胞吸收的砷与磷酸盐之间的关系方面，研究了植物如何通过砷暴露诱导含硫醇（SH）化合物和蛋白质的机制。蜈蚣草愈伤组织可通过快速引入还原酶将五价砷有效还原为三价砷，并合成硫醇导致植物螯合肽的产生。此外，蜈蚣草可根据亚砷酸盐和砷酸盐的浓度控制细胞内的磷酸盐浓度。据我们所知，这是第一份利用愈伤组织，从体外分析砷形态和代谢途径的角度，展示蜈蚣草对高浓度砷具有耐受性的机制的报告。
