[高效液相色谱-电化学检测法研究大鼠血浆中黄芩苷和黄芩素的稳定性]
[Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD].
作者信息
Wang Qi, Zhang Yu-jie, Li Wei-feng, Yang Jie, Jiang Hao, Sun Wan-jing
机构信息
School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2675-8.
OBJECTIVE
To compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.
METHOD
HPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them.
RESULT
Baicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%.
CONCLUSION
The stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.
目的
比较黄芩苷和黄芩素在缓冲水溶液及小鼠血浆中的稳定性，以探讨其降解机制及样品处理方法。
方法
采用高效液相色谱 - 电化学检测法（HPLC - ECD）测定不同条件下的黄芩苷和黄芩素，并选择样品处理方法以使其稳定。
结果
黄芩苷和黄芩素在pH 7.4的缓冲水溶液中比在小鼠血浆中更稳定。在缓冲水溶液中加入维生素C后，黄芩苷和黄芩素的稳定性可明显提高，其残留率分别为102%和100%。仅在小鼠血浆中加入维生素C和1 mol·L⁻¹盐酸时，黄芩苷和黄芩素的稳定性可显著增强，其残留率分别高达98%和103%。
结论
黄芩苷和黄芩素的稳定性与氧化及酶降解有关，可通过在溶液中加入维生素C和1 mol·L⁻¹盐酸来提高。
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