[大骨节病和骨关节炎患者滑膜及滑液中白细胞介素-1β和肿瘤坏死因子-α的表达]
[Expression of interleukin-1beta and tumor necrosis factor-alpha in the synovium and synovial fluid of patients with Kashin-Beck disease and osteoarthritis].
作者信息
Sun Zheng-ming, Ling Ming, Liu Miao, Zhang Yin-gang
机构信息
Department of Orthopedics, First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Jan;29(1):5-8.
OBJECTIVE
To investigate the role of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in the pathogenesis and progression of Kashin-Beck disease (KBD) and primary osteoarthritis (OA).
METHODS
The synovium and synovial fluid of the knee joint were collected from 20 adult patients with KBD, 18 with OA and 19 with meniscus injury (controls). The expression of IL-1beta and TNF-alpha in the synovium were analyzed by immunohistochemistry staining, and the levels of IL-1beta and TNF-alpha in the synovial fluid were determined by enzyme-linked immunosorbent assay.
RESULT
IL-1beta and TNF-alpha expressions in the synovium and their levels in the synovial fluid were significantly higher in patients with KBD and OA than in patients with meniscus injury (P<0.05), but comparable between KBD and OA groups (P>0.05).
CONCLUSION
IL-1beta and TNF-alpha may play an important role in the pathogenesis and progression of KBD and OA.
目的
探讨白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）在大骨节病（KBD）和原发性骨关节炎（OA）发病机制及病情进展中的作用。
方法
收集20例成年KBD患者、18例OA患者和19例半月板损伤患者（对照组）的膝关节滑膜及滑液。采用免疫组织化学染色分析滑膜中IL-1β和TNF-α的表达，采用酶联免疫吸附测定法测定滑液中IL-1β和TNF-α的水平。
结果
KBD和OA患者滑膜中IL-1β和TNF-α的表达及其滑液中的水平显著高于半月板损伤患者（P<0.05），但KBD组和OA组之间相当（P>0.05）。
结论
IL-1β和TNF-α可能在KBD和OA的发病机制及病情进展中起重要作用。
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