通过模板化介孔二氧化硅球制备生物相容性多肽微胶囊

Biocompatible polypeptide microcapsules via templating mesoporous silica spheres.

作者信息

Yu Aimin, Gentle Ian R, Lu Gao Qing Max

机构信息

ARC Centre of Excellence for Functional Nanomaterials, The University of Queensland, 4072, Australia.

出版信息

J Colloid Interface Sci. 2009 May 1;333(1):341-5. doi: 10.1016/j.jcis.2009.01.036. Epub 2009 Jan 24.

DOI:10.1016/j.jcis.2009.01.036

PMID:19223040

Abstract

We reported the stepwise formation of biocompatible poly(L-lysine)/poly(L-glutamic acid) (PLL/PGA) multilayer films on mesoporous silica (MS) spheres via layer-by-layer (LbL) self-assembly technique. In-situ QCM revealed the nonlinear (exponential) growth of PLL/PGA multilayer films at both pH 5.5 and pH 7.0 conditions. Xi-potential measurements of the multilayer coated particles indicated that the multilayer surface was being charge-overcompensated in each adsorption step, thereby facilitating adsorption of the next oppositely charged polypeptide onto the MS spheres. Hollow polypeptide capsules could be obtained by subsequently removing silica cores in HF solution. By using enzyme-preloaded MS spheres as capsule templates, a general approach was developed for encapsulating enzymes in biocompatible microcapsules with high loading and retained bioactivity. The loading amount for several enzymes with different sizes and their bioactivity after encapsulation were also reported.

摘要

我们报道了通过层层（LbL）自组装技术在介孔二氧化硅（MS）球上逐步形成生物相容性聚（L-赖氨酸）/聚（L-谷氨酸）（PLL/PGA）多层膜。原位石英晶体微天平（QCM）显示，在pH 5.5和pH 7.0条件下，PLL/PGA多层膜均呈非线性（指数）生长。对多层包覆颗粒的ζ电位测量表明，在每个吸附步骤中多层表面电荷都被过度补偿，从而有利于下一个带相反电荷的多肽吸附到MS球上。随后通过在氢氟酸（HF）溶液中去除二氧化硅核，可以得到中空的多肽胶囊。通过使用预载酶的MS球作为胶囊模板，开发了一种将酶封装在具有高负载量和保留生物活性的生物相容性微胶囊中的通用方法。还报道了几种不同大小的酶的负载量及其封装后的生物活性。