日本正常人群亚端粒甲基化的年龄相关变化。
Age-related changes in subtelomeric methylation in the normal Japanese population.
作者信息
Maeda Toyoki, Guan Jing Zhi, Oyama Jun-ichi, Higuchi Yoshihiro, Makino Naoki
机构信息
Division of Molecular and Clinical Gerontology, Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, Beppu, Oita 874-0838, Japan.
出版信息
J Gerontol A Biol Sci Med Sci. 2009 Apr;64(4):426-34. doi: 10.1093/gerona/gln057. Epub 2009 Feb 17.
BACKGROUND
The telomeres of somatic cells become shorter with individual aging. However, no significant change in subtelomeric methylation of somatic cells with aging has yet been reported.
METHODS
Telomere lengths of the peripheral blood cells of 148 normal Japanese were analyzed by Southern blotting using methylation-sensitive and -insensitive isoschizomers.
RESULTS
With aging, long telomeres decrease and short telomeres increase, and the contents of the telomeres with methylated subtelomere increase in long telomeres, thus leading us to postulate that telomeres with less methylated subtelomeres tend to become shortened faster.
CONCLUSIONS
A telomere length distribution analysis with methylation-sensitive and -insensitive isoschizomer seems to be a useful tool to assess the subtelomeric methylation status of the somatic cell population. The subtelomeric methylation of peripheral blood cells is also indicated to be an indicator for aging-associated genomic changes.
背景
体细胞的端粒会随着个体衰老而变短。然而，目前尚无关于体细胞亚端粒甲基化随衰老发生显著变化的报道。
方法
使用甲基化敏感和不敏感的同裂酶，通过Southern印迹法分析了148名正常日本人群外周血细胞的端粒长度。
结果
随着衰老，长端粒减少，短端粒增加，并且长端粒中具有甲基化亚端粒的端粒含量增加，从而使我们推测亚端粒甲基化程度较低的端粒往往缩短得更快。
结论
使用甲基化敏感和不敏感同裂酶进行端粒长度分布分析似乎是评估体细胞群体亚端粒甲基化状态的有用工具。外周血细胞的亚端粒甲基化也被表明是衰老相关基因组变化的一个指标。
Zotero 插件