使用转棒试验作为大鼠行走定性分析的一种方法。
Use of rotorod as a method for the qualitative analysis of walking in rat.
作者信息
Whishaw Ian Q, Li Katie, Whishaw Paul A, Gorny Bogdan, Metz Gerlinde A
机构信息
Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge.
出版信息
J Vis Exp. 2008 Dec 10(22):1030. doi: 10.3791/1030.
High speed video analysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a rotorod, animals remain in approximately the same place making repetitive movements of stepping. Thus the task provides a rich source of information on the details of foot stepping movements. Subjects were hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the right nigrostriatal bundle to deplete nigrostriatal dopamine (DA). The present report provides a video analysis illustration of animals previously were filmed from frontal, lateral, and posterior views as they walked (15). Rating scales and frame-by-frame replay of the video records of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in posture and limb use contralateral to the DA-depletion. The contralateral limbs participated less in initiating and sustaining propulsion than the ipsilateral limbs.These deficits secondary to unilateral DA-depletion show that the rotorod provides a use task for the analysis of stepping movements.A more detailed presentation of the present study has been made (Whishaw et al, 2003), but the present study presents the video support describing the stepping movement in the good and affected limbs of unilateral dopamine-depleted rats. For the analysis, rats with unilateral DA depletions and control rats were video recorded from front, lateral and posterior views. A rating scale of posture and forelimb movements indicated that stepping movements were chronically impaired following surgery. Examination of limb movements indicated that whereas the DA-depleted rats could use the limbs contralateral to the lesion for support, they received minimal use for shifting weight. The results of this study indicate that the rotorod task, in addition to providing quantitative measures of motor impairments, can also provide insights into the qualitative impairments [corrected].
对运动细节进行高速视频分析可为步行运动的定性方面提供信息来源。当动物在转棒上行走时，它们会停留在大致相同的位置，进行重复的踏步动作。因此，该任务为足部踏步运动的细节提供了丰富的信息来源。实验对象是半帕金森样大鼠，通过向右侧黑质纹状体束注射6-羟基多巴胺（6-OHDA）以耗尽黑质纹状体多巴胺（DA）来制备。本报告提供了对动物的视频分析例证，这些动物在行走时从前、侧和后视图进行拍摄（15）。对踏步行为的视频记录进行评分量表和逐帧回放表明，半帕金森大鼠在与DA耗竭对侧的姿势和肢体使用方面长期受损。对侧肢体在启动和维持推进方面比同侧肢体参与更少。单侧DA耗竭导致的这些缺陷表明，转棒为分析踏步运动提供了一个实用任务。本研究已进行了更详细的介绍（惠肖等人，2003年），但本研究提供了视频支持，描述了单侧多巴胺耗竭大鼠正常和受影响肢体的踏步运动。为了进行分析，对单侧DA耗竭的大鼠和对照大鼠从前、侧和后视图进行视频记录。姿势和前肢运动的评分量表表明，手术后踏步运动长期受损。对肢体运动的检查表明，虽然DA耗竭的大鼠可以使用与损伤对侧的肢体进行支撑，但它们在转移体重方面的使用最少。本研究结果表明，转棒任务除了提供运动损伤的定量测量外，还可以深入了解定性损伤[已修正]。
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