人脐带血细胞体外形成毛细血管样小管及其治疗应用前景
In vitro formation of capillary tubules from human umbilical cord blood cells with perspectives for therapeutic application.
作者信息
Senegaglia Alexandra Cristina, Brofman Paulo Roberto Slud, Aita Carlos Alberto Mayora, Dallagiovanna Bruno, Rebelatto Carmen Lúcia Kuniyoshi, Hansen Paula, Barchiki Fabiane, Krieger Marco Aurélio
机构信息
Núcleo de Cardiomioplastia Celular, Pontifícia Universidade Católica do Paraná.
出版信息
Rev Bras Cir Cardiovasc. 2008 Oct-Dec;23(4):467-73. doi: 10.1590/s0102-76382008000400003.
OBJECTIVE
Endothelial progenitor cells (EPC) characterized by the CD133+ marker, contribute to the neovascularization. Increasing EPC number in vitro could be a promising therapeutic tool. Human umbilical cord blood maintains a significant number of EPC, suggesting the possibility to use these cells to induce the revascularization of ischemic tissues. The aim of this study was to analyze the in vitro function of differentiated CD133+ cells.
METHODS
Cells were characterized by flow cytometry, VEGF mRNA expression was evaluated by the RT-PCR analysis and the functionally by essays of capillary tubes formation.
RESULTS
Differentiated cells lost EPC markers, maintained low levels of markers for hematopoietic and monocytic cell lines and increased the expression of adult endothelial cell markers. Differentiated cells expressed VEGF mRNA and were capable to induce in vitro capillary tubules formation.
CONCLUSION
CD133+ cells differentiated into endothelial cells in vitro are functionally active initiating the possibility of their use in future therapeutic applications.
目的
以CD133+为标志物的内皮祖细胞（EPC）有助于新血管形成。在体外增加EPC数量可能是一种有前景的治疗手段。人脐带血中含有大量EPC，这提示了利用这些细胞诱导缺血组织血管再生的可能性。本研究的目的是分析分化的CD133+细胞的体外功能。
方法
通过流式细胞术对细胞进行鉴定，采用逆转录-聚合酶链反应（RT-PCR）分析评估VEGF mRNA表达，并通过毛细管形成试验进行功能评估。
结果
分化后的细胞失去了EPC标志物，造血和单核细胞系标志物水平维持在较低水平，而成人内皮细胞标志物的表达增加。分化后的细胞表达VEGF mRNA，并且能够在体外诱导毛细管形成。
结论
体外分化为内皮细胞的CD133+细胞具有功能活性，这为其在未来治疗应用中的使用开启了可能性。
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