牛磺酸、次牛磺酸及牛磺酸同系物对大鼠因糖尿病、酒精中毒及糖尿病合并酒精中毒所致红细胞形态、膜流动性及细胞骨架血影蛋白改变的影响。

The effects of taurine, hypotaurine, and taurine homologs on erythrocyte morphology, membrane fluidity and cytoskeletal spectrin alterations due to diabetes, alcoholism and diabetes-alcoholism in the rat.

作者信息

Gossai Davekanand, Lau-Cam Cesar A

机构信息

Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, St. John's University, Jamaica, New York, USA.

出版信息

Adv Exp Med Biol. 2009;643:369-79. doi: 10.1007/978-0-387-75681-3_38.

DOI:10.1007/978-0-387-75681-3_38

PMID:19239168

Abstract

Taurine (TAU) and compounds representing a TAU analog (hypotaurine = HYTAU) or homolog (aminomethanesulfonic acid = AMSA, homotaurine = HMTAU) were tested for their counteracting effects against alterations in erythrocyte (RBC) morphology, membrane fluidity and cytoskeletal spectrin distribution due to diabetes, alcoholism and diabetes-alcoholism in male Goto-Kakizaki rats (made diabetic with a high fat diet and alcoholic upon feeding on a flavored alcohol solution) and Wistar-Kyoto rats (serving as controls). Both diabetes and alcoholism changed the RBC discoidal biconcave shape to a spiculated one, lowered membrane fluidity, and caused spectrin to become marginalized. While AMSA and HYTAU returned the RBC shape to normal, HMTAU made it only discoidal, and TAU was without effect. All test compounds, but TAU, maintained the membrane fluidity normal; and HYTAU and AMSA, but not TAU or HMTAU, kept spectrin uniformly distributed. The noted effects were correlated with compound structure and RBC values for malondialdehyde and cholesterol/phospholipid ratio.

摘要

在雄性五岛-加崎大鼠（通过高脂饮食诱导糖尿病，并在喂食调味酒精溶液后诱导酒精成瘾）和Wistar-Kyoto大鼠（作为对照）中，测试了牛磺酸（TAU）以及代表TAU类似物（亚牛磺酸=HYTAU）或同系物（氨基甲磺酸=AMSA、高牛磺酸=HMTAU）的化合物对因糖尿病、酒精成瘾以及糖尿病合并酒精成瘾导致的红细胞（RBC）形态、膜流动性和细胞骨架血影蛋白分布改变的对抗作用。糖尿病和酒精成瘾均使红细胞的盘状双凹形变为棘状，降低了膜流动性，并导致血影蛋白边缘化。虽然AMSA和HYTAU使红细胞形状恢复正常，但HMTAU仅使其变为盘状，而TAU则无作用。除TAU外，所有测试化合物均使膜流动性保持正常；而HYTAU和AMSA（而非TAU或HMTAU）使血影蛋白均匀分布。上述作用与化合物结构以及红细胞丙二醛值和胆固醇/磷脂比值相关。