Gafken Philip R
Proteomics Facility, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Methods Mol Biol. 2009;527:159-72, ix. doi: 10.1007/978-1-60327-834-8_12.
Protein phosphorylation is a reversible and frequently occurring posttranslational modification regulating a large number of biological functions. Understanding the role phosphorylation events play in biochemical pathways requires the detection of phosphorylated proteins and their phosphorylated amino acids. Mass spectrometry has developed as the premier method for characterizing phosphoproteins as it is sensitive to detecting phosphosites within a single protein or a complex protein mixture (phosphoproteomics). Here an overview is provided of the sample separation and mass spectrometry techniques commonly used for qualitative phosphoprotein analysis.
蛋白质磷酸化是一种可逆且频繁发生的翻译后修饰，可调节大量生物学功能。要了解磷酸化事件在生化途径中所起的作用，需要检测磷酸化蛋白及其磷酸化氨基酸。质谱分析法已发展成为表征磷酸化蛋白的首要方法，因为它能够灵敏地检测单个蛋白或复杂蛋白混合物中的磷酸化位点（磷酸化蛋白质组学）。本文概述了常用于磷酸化蛋白定性分析的样品分离和质谱分析技术。
