外源性鼠疫耶尔森菌群体感应分子N-辛酰基高丝氨酸内酯和N-（3-氧代辛酰基）高丝氨酸内酯调节LcrV毒力因子。
Exogenous Yersinia pestis quorum sensing molecules N-octanoyl-homoserine lactone and N-(3-oxooctanoyl)-homoserine lactone regulate the LcrV virulence factor.
作者信息
Gelhaus H Carl, Rozak David A, Nierman William C, Chen Dan, Varga John J, Zadeh Mojgan, Ulrich Ricky L, Adamovicz Jeffrey J
机构信息
United States Army Medical Institute of Infectious Diseases, Bacteriology Division, 1425 Porter St., 21702 Ft. Detrick, MD, USA.
出版信息
Microb Pathog. 2009 May;46(5):283-7. doi: 10.1016/j.micpath.2009.02.002. Epub 2009 Feb 26.
LcrV is a key Yersinia pestis antigen, immune regulator, and component of the type III secretion system (T3SS). Researchers have shown that N-acyl-homoserine lactones (AHLs) can down-regulate the expression of the LcrV homolog, PcrV, in Pseudomonas aeruginosa. Using ELISA, western blot, DNA microarray analysis, and real time PCR we demonstrate that the addition of AHL molecules N-octanoyl-homoserine lactone (C8) or N-(3-oxooctanoyl)-homoserine lactone (oxo-C8) to Y. pestis cultures down-regulates LcrV protein expression. DNA microarray analysis shows 10 additional T3SS genes are consistently down-regulated by C8 or oxo-C8. This is the first report demonstrating that AHLs regulate Y. pestis virulence factor expression.
LcrV是鼠疫耶尔森菌的一种关键抗原、免疫调节剂以及III型分泌系统（T3SS）的组成部分。研究人员已表明，N-酰基高丝氨酸内酯（AHLs）可下调铜绿假单胞菌中LcrV同源物PcrV的表达。我们通过酶联免疫吸附测定（ELISA）、蛋白质免疫印迹法、DNA微阵列分析和实时聚合酶链反应（PCR）证明，向鼠疫耶尔森菌培养物中添加AHL分子N-辛酰高丝氨酸内酯（C8）或N-（3-氧代辛酰基）高丝氨酸内酯（oxo-C8）可下调LcrV蛋白表达。DNA微阵列分析表明，另外10个T3SS基因也会持续被C8或oxo-C8下调。这是首份证明AHLs可调节鼠疫耶尔森菌毒力因子表达的报告。
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