Itsko Mark, Rabinovitch Avinoam, Zaritsky Arieh
Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva 84105, Israel.
Biophys J. 2009 Mar 4;96(5):1866-74. doi: 10.1016/j.bpj.2008.10.061.
Repetitive DNA is a periodic copolymer with the intrinsic property of exponential propagation to longer repeats. Microgene polymerization reaction (MPR) is a model system in which a short nonrepetitive homo-duplex DNA evolves to multiple repetitive products during heat-cool cycles. The mechanism underlying this process involves staggered annealing of complementary DNA strands of variable lengths and polymerase-mediated filling-in of the generated overhangs. MPR is considered here as a process sharing common features with two polymerization types, chain-growth and step-growth, and significant distinctions from both types were highlighted. The involved reaction stages were formulated and a kinetic model was derived and tested experimentally. The model can quantitatively explain MPR propagation and be used as a good approximation for this phenomenon.
重复DNA是一种具有指数级传播至更长重复序列内在特性的周期性共聚物。微基因聚合反应（MPR）是一种模型系统，在该系统中，短的非重复同源双链DNA在热循环冷却过程中演变成多个重复产物。这一过程的潜在机制涉及不同长度互补DNA链的交错退火以及聚合酶介导的对产生的突出端的填补。本文将MPR视为一个与链增长和逐步增长这两种聚合类型具有共同特征的过程，并突出了与这两种类型的显著区别。阐述了所涉及的反应阶段，推导了动力学模型并进行了实验测试。该模型可以定量解释MPR的传播，并可作为对这一现象的良好近似。
