[从外科重症监护病房分离出的多重耐药铜绿假单胞菌菌株的基因组比较]

[Genomic comparisons of multiresistant Pseudomonas aeruginosa isolates obtained from surgical intensive care unit].

作者信息

Wang Yu-Ming, Liu Yu-Cun, Wan Yuan-Lian, Liu Shu-Lin, Liu Gui-Rong, Wang Xin, Wang Qiu-Shi, Cao Xiao-Li

机构信息

Department of General Surgery, Peking University First Hospital, Beijing 100034, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2008 Dec 23;88(47):3319-24.

PMID:19257960

Abstract

OBJECTIVE

To investigate the genetic differences and their relativity with multi-drug resistance of Pseudomonas aeruginosa isolated.

METHODS

Forty-nine Pseudomonas aeruginosa isolates were characterized by antimicrobial susceptibility and four-enzyme (I-CeuI, SpeI, SwaI, PacI) pulsed-field gel electrophoresis (PFGE).

RESULTS

85.7% of the P.aeruginosa isolates were MDR strains. Strains with PFGE pattern A were all susceptible to amikacin and cefepime, but were resistant to levofloxacin and meropenem. Strains with PFGE Patterns H and P had resistance to 6 - 8 different kinds of antibiotics. Strains with PFGE Patterns I and J were susceptible to all antibiotics tested in this study. Strains with other PFGE Patterns had intermediate resistance. PFGE pattern A was the dominant pattern, which accounted for 61.2% of all P.aeruginosa strains, 100% (2/2) in 2001, 65% (13/20) in 2002, 44.4% (8/18) in 2003 and 77.8% (7/9) in 2004.

CONCLUSION

Four-enzyme combined PFGE analysis is highly discriminatory for the subtyping of MDR P.aeruginosa isolates.

摘要

目的

研究分离出的铜绿假单胞菌的基因差异及其与多重耐药性的相关性。

方法

采用抗菌药物敏感性试验和四种酶（I-CeuI、SpeI、SwaI、PacI）脉冲场凝胶电泳（PFGE）对49株铜绿假单胞菌进行分型。

结果

85.7%的铜绿假单胞菌分离株为多重耐药菌株。PFGE图谱为A型的菌株对阿米卡星和头孢吡肟均敏感，但对左氧氟沙星和美罗培南耐药。PFGE图谱为H型和P型的菌株对6 - 8种不同抗生素耐药；PFGE图谱为I型和J型的菌株对本研究中所检测的所有抗生素均敏感；其他PFGE图谱类型的菌株为中度耐药。PFGE图谱A为优势图谱，占所有铜绿假单胞菌菌株的61.2%，2001年为100%（2/2），2002年为65%（13/20），2003年为44.4%（8/18），2004年为77.8%（7/9）。