Suzuki M, Kitamura K, Nomura Y
Department of Otolaryngology, Tokyo Metropolitan Bokutou Hospital, Japan.
Acta Otolaryngol Suppl. 1991;481:112-5. doi: 10.3109/00016489109131360.
The presence of anionic sites in the labyrinth is demonstrated in this study, using polyethyleneimine as a cationic probe. Hartley-strain guinea pigs (200-300 g) with a normal Preyer's reflex were used. A 0.5% polyethyleneimine (PEI, MW 1,800) solution adjusted to pH 7.3 with HCl following Schurer's method was systemically administered through an axillary vein. In the cochlea, the presence of anionic sites was demonstrated on the basement membrane of the capillary wall in the stria vascularis. The presence of anionic sites was also confirmed on the basement membrane in Reissner's membrane but its density was much lower than that in the stria vascularis. In the ampulla and macula, the anionic sites were present on the basement membrane of the capillary wall and sensory epithelium.
本研究使用聚乙烯亚胺作为阳离子探针，证实了内耳迷路中阴离子位点的存在。选用具有正常普赖尔反射的哈特利品系豚鼠（200 - 300克）。按照舒勒方法，用盐酸将0.5%聚乙烯亚胺（PEI，分子量1800）溶液的pH值调至7.3，通过腋静脉进行全身给药。在耳蜗中，血管纹毛细血管壁的基底膜上证实有阴离子位点存在。在Reissner膜的基底膜上也证实有阴离子位点存在，但其密度远低于血管纹中的密度。在壶腹和黄斑中，阴离子位点存在于毛细血管壁和感觉上皮的基底膜上。
