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来自两大洲的球孢囊线虫核糖体DNA比较。

Ribosomal DNA comparisons of globodera from two continents.

作者信息

Ferris V R, Miller L I, Faghihi J, Ferris J M

出版信息

J Nematol. 1995 Sep;27(3):273-83.

Abstract

Ribosomal DNA (rDNA) sequence data were compared for five species of Globodera, including G. rostochiensis, G. pallida, G. virginiae, and two undescribed Globodera isolates from Mexico collected from weed species and maintained on Solanum dulcamara. The rDNA comparisons included both internal transcribed spacers (ITS1 and ITS2), the 5.8S rRNA gene, and small portions of the 3' end of the 18S gene and the 5' end of the 28S gene. Phylogenetic analysis of the rDNA sequence data indicated that the two potato cyst nematodes, G. pallida and especially G. rostochiensis, are closely related to the Mexican isolates, whereas G. virginiae is relatively dissimilar to the others and more distantly related. The data are consistent with the thesis that Mexico is the center of origin for the potato cyst nematodes.

摘要

对五种球孢囊线虫的核糖体DNA(rDNA)序列数据进行了比较,其中包括罗斯托克球孢囊线虫、苍白球孢囊线虫、弗吉尼亚球孢囊线虫,以及从杂草物种中采集并保存在苦甜茄上的来自墨西哥的两种未描述的球孢囊线虫分离株。rDNA比较包括内部转录间隔区(ITS1和ITS2)、5.8S rRNA基因,以及18S基因3'端和28S基因5'端的小部分。rDNA序列数据的系统发育分析表明,两种马铃薯孢囊线虫,即苍白球孢囊线虫,尤其是罗斯托克球孢囊线虫,与墨西哥分离株密切相关,而弗吉尼亚球孢囊线虫与其他线虫相对不同,亲缘关系更远。这些数据与墨西哥是马铃薯孢囊线虫起源中心的论点一致。

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