Lochmatter C, Schifferli J A, Martin P J
Department of Biomedicine, Immunonephrology, University Hospital Basel, Basel, Switzerland.
Parasitology. 2009 Apr;136(5):487-98. doi: 10.1017/S0031182009005757. Epub 2009 Mar 13.
A trispanning orphan receptor (TOR) has been described in Schistosoma haematobium and S. mansoni. Here we report the complete molecular organization of the S. mansoni TOR gene, also known as SmCRIT (complement C2 receptor inhibitor trispanning). The SmTOR gene consists of 4 exons and 3 introns as shown by cloning the single exons from S. mansoni genomic DNA and the corresponding cDNA from the larval stage (cercaria) and the adult worm. The SmTOR ORF consists of 1260 bp and is longer than previously reported, with a fourth trans-membrane domain (proposed new name: Tetraspanning Orphan Receptor) and with, however, an unchanged C2-binding domain on the extracellular domain 1 (ed1). This domain differs in S. japonicum. A protein at the approximate expected molecular weight (55 kDa) was detected in adult worm extracts with polyclonal and monoclonal antibodies, and was found to be expressed on the tegumental surface of cercariae.
在埃及血吸虫和曼氏血吸虫中已发现一种三跨膜孤儿受体（TOR）。在此，我们报道曼氏血吸虫TOR基因的完整分子结构，该基因也被称为SmCRIT（补体C2受体抑制剂三跨膜蛋白）。通过从曼氏血吸虫基因组DNA中克隆单个外显子以及从幼虫期（尾蚴）和成虫中克隆相应的cDNA，结果显示SmTOR基因由4个外显子和3个内含子组成。SmTOR开放阅读框（ORF）由1260个碱基对组成，比之前报道的更长，具有第四个跨膜结构域（提议的新名称：四跨膜孤儿受体），然而，其细胞外结构域1（ed1）上的C2结合结构域未发生变化。该结构域在日本血吸虫中有所不同。用多克隆抗体和单克隆抗体在成虫提取物中检测到一种分子量约为预期值（55 kDa）的蛋白质，并且发现该蛋白质在尾蚴的体表表达。
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