Hassan Sedky H A, Kim Su-Jung, Jung A-Young, Joo Jin Ho, Eun Oh Sang, Yang Jae E
Department of Biological Environment, Kangwon National University, Chuncheon, Korea.
J Gen Appl Microbiol. 2009 Feb;55(1):27-34. doi: 10.2323/jgam.55.27.
The biosorptive capacity of Cd(II) and Cu(II) by lyophilized cells of Pseudomonas stutzeri was investigated based on Langmuir and Freundlich isotherms and biosorption kinetics were analyzed using first order kinetic with different initial metal concentrations. Biosorptive capacity for Cd(II) and Cu(II) decreased with an increment of metal concentration, reaching 43.5 and 36.2 mg/g at the initial concentration of 300 mg/L. Biosorption capacity for both metal ions was increased with increasing pH. The optimum pH for biosorption rate of Cu(II) and Cd(II) was pH 5; above pH 5.0 the metal cations came to be precipitated. The experimental data showed a better fit with the Langmuir model over the Freundlich model for both metal ions throughout the range of initial concentrations. The maximum sorptive capacity (q max) obtained from the Langmuir equation for Cd(II) and Cu(II) were 47.86 (r(2)=0.99) and 33.16 (r(2)=0.99), respectively. The bacterial cells have more affinity to adsorb cadmium than copper. The first order kinetic was well fitted to the experimental data for initial concentrations from 30 to 100 mg/L during reaction times of 250 min. These results suggest that biosorption of Cu(II) and Cd(II) by lyophilized cells of P. stutzeri is a potential metal removal strategy.
基于朗缪尔(Langmuir)等温线和弗伦德利希(Freundlich)等温线,研究了施氏假单胞菌冻干细胞对镉(II)和铜(II)的生物吸附能力,并使用一级动力学分析了不同初始金属浓度下的生物吸附动力学。镉(II)和铜(II)的生物吸附能力随金属浓度的增加而降低,在初始浓度为300 mg/L时分别达到43.5和36.2 mg/g。两种金属离子的生物吸附能力均随pH值的升高而增加。铜(II)和镉(II)生物吸附速率的最佳pH值为5;pH值高于5.0时,金属阳离子开始沉淀。在整个初始浓度范围内,实验数据表明两种金属离子与朗缪尔模型的拟合度优于弗伦德利希模型。由朗缪尔方程得到的镉(II)和铜(II)的最大吸附容量(q max)分别为47.86(r(2)=0.99)和33.16(r(2)=0.99)。细菌细胞对镉的吸附亲和力大于铜。在250分钟的反应时间内,一级动力学很好地拟合了初始浓度为30至100 mg/L的实验数据。这些结果表明,施氏假单胞菌冻干细胞对铜(II)和镉(II)的生物吸附是一种潜在的金属去除策略。